The Cultrex® Spheroid Invasion Matrix and Plate utilize a 3D Culture Qualified 96 Well Spheroid Formation Plate to direct spheroid formation for cells that do not require exogenous extracellular matrix. Upon completion of spheroid formation, the spheroid is embedded in Invasion Matrix composed of basement membrane and collagen proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.