Cultrex® 3D Spheroid Colorimetric Proliferation/Viability Assay

The Cultrex® 3D Spheroid Colorimetric Proliferation/Viability Assay provides a powerful tool for modeling tumor growth response in vitro using colorimetric detection. Tumor cell aggregates, or spheroids, are more representative of tumors in vivo, and they exhibit several physiological traits including similar morphology, formation of cell-cell bonds, decreased proliferation rates, increased cell survival, tumor dormancy, and a hypoxic core. Applying this model to in a 3D culture cell proliferation and viability assay provides a more physiological approach for objectively quantifying tumor growth, also providing a visual component that can be quantified through image analysis.

Available Size(s): 96 Samples Catalog Number: 3511-096-K Categories: ,

Description

The Cultrex® 3D Spheroid Colorimetric Proliferation/Viability Assay utilizes a 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid may be treated with pharmacological agents to evaluate tumor viability after drug treatment. Tumor spheroid expansion is visualized microscopically and can be quantified through image analysis software for real-time and label free evaluation. At the conclusion of the assay, cell viability may be assessed by absorbance using MTT. The 3D Spheroid Colorimetric Proliferation/Viability Assay offers an in vitro, standardized, three-dimensional, high content format for inducing multicellular tumor spheroid (MCTS) formation and quantitifying cell viability within the spheroids in response to pharmacological treatment.

 

Protocol(s)

protocol_3511-096-K

Material Safety Data Sheet(s)

msds_3500-096-01 10X Spheroid Formation ECM

msds_3511-096-01 MTT Reagent

msds_3511-096-02 Detergent Reagent

3500-096-02 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3511-096-K includes :
Catalog Number Description Qty
3500-096-01 Cultrex® Spheroid Formation Matrix 1
3500-096-02 3D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals 1
3511-096-01 MTT Reagent 1
3511-096-02 Detergent Reagent 1
Cultrex® 3D Spheroid Colorimetric Proliferation Assay.
Cultrex® 3D Spheroid Colorimetric Proliferation Assay Diagram.
Cultrex® 3D Spheroid Colorimetric Proliferation Assay Data.