Cultrex® 3D Spheroid Cell Invasion Assay


The Cultrex® 3D Culture Spheroid Cell Invasion Assay provides a more complete and physiologically predictive cancer invasion model.  Tumor cell aggregates, or spheroids, are more representative of tumors in vivo, and they exhibit several physiological traits including similar morphology, formation of cell-cell bonds, decreased proliferation rates, increased cell survival, tumor dormancy, and a hypoxic core. Applying this model to in a 3D culture cell invasion assay provides a more physiological approach for assessing tumor invasion, also providing a visual component that can be quantified through image analysis.

Available Size(s): 96 Samples Catalog Number: 3500-096-K Categories: ,


The Cultrex® 3D Culture Spheroid Cell Invasion Assay utilizes a 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells. Upon completion of spheroid formation, the spheroid is embedded in an invasion matrix composed of basement membrane proteins. This matrix forms a hydrogel network on which invasive cells can travel. Invasion modulating agents can be applied to the system to evaluate the impact on cell response. Cell invasion is visualized microscopically and can be quantified through image analysis software.



Material Safety Data Sheet(s)

msds_3500-096-03 Invasion Matrix

msds_3500-096-01 10X Spheroid Formation ECM

3500-096-02 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3500-096-K includes :
Catalog Number Description Qty
3500-096-01 Cultrex® Spheroid Formation Matrix 1
3500-096-02 3D Culture Qualified 96 Well Spheroid Formation Plate with Strip Seals 1
3500-096-03 Cultrex® Spheroid Invasion Matrix 1

Cultrex® 3D Spheroid Cell Invasion Assay.

Cultrex® 3D Spheroid Invasion Diagram.
Cultrex® 3D Spheroid Invasion Over Time.
Cultrex® 3D Spheroid Invasion Inhibition.
Cultrex® Spheroid Invasion for Different Cell Lines.

Abstract 2033: A comprehensive 3D triple coculture model for evaluating breast cancer progression
Gabriel J. Benton, Jay George, Gerald DeGray, Irina Arnaoutova, and Hynda K. Kleinman
Cancer Res., Oct 2014; 74: 2033.}

Endophilin A2 Promotes TNBC Cell Invasion and Tumor Metastasis
Tomas Baldassarre, Kathleen Watt, Peter Truesdell, Jalna Meens, Mark M. Schneider, Sandip K. Sengupta, and Andrew W. Craig
Mol. Cancer Res., Jun 2015; 13: 1044 – 1055.

Binding of 14-3-3 reader proteins to phosphorylated DNMT1 facilitates aberrant DNA methylation and gene expression
Pierre-Olivier Estève, Guoqiang Zhang, V.K. Chaithanya Ponnaluri, Kanneganti Deepti, Hang Gyeong Chin, Nan Dai, Cari Sagum, Karynne Black, Ivan R. Corrêa, Jr., Mark T. Bedford, Xiaodong Cheng, and Sriharsa Pradhan
Nucleic Acids Res., Nov 2015; 10.1093/nar/gkv1162.

CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma
Sun Wook Cho, Young A Kim, Hyun Jin Sun, Ye An Kim, Byung-Chul Oh, Ka Hee Yi, Do Joon Park, and Young Joo Park
Endocr. Relat. Cancer, Dec 2015; 23: 113 – 124.

The oncogenic triangle of HMGA2, LIN28B and IGF2BP1 antagonizes tumor-suppressive actions of the let-7 family
Bianca Busch, Nadine Bley, Simon Müller, Markus Glaß, Danny Misiak, Marcell Lederer, Martina Vetter, Hans-Georg Strauß, Christoph Thomssen, and Stefan Hüttelmaier
Nucleic Acids Res., May 2016; 44: 3845 – 3864.Nucleic Acids Res., May 2016; 44: 3845 – 3864.

“Tumor Cell Invasion Can Be Blocked by Modulators of Collagen Fibril
Alignment That Control Assembly of the Extracellular Matrix”

“Moran Grossman, Nir Ben-Chetrit, Alina Zhuravlev, Ran Afik, Elad Bassat, Inna Solomonov, Yosef Yarden,
and Irit Sagi”
Cancer Res., Jul 2016; 76: 4249 – 4258.

“RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs”
“Hiroaki Kajiho, Yuko Kajiho, Emanuela Frittoli, Stefano Confalonieri, Giovanni Bertalot, Giuseppe Viale,
Pier Paolo Di Fiore, Amanda Oldani, Massimiliano Garre, Galina V Beznoussenko, Andrea Palamidessi,
Manuela Vecchi, Philippe Chavrier, Frank Perez, and Giorgio Scita”
EMBO Rep., Jul 2016; 17: 1061 – 1080.

The GAS6-AXL signaling network is a mesenchymal (Mes) molecular subtype–specific therapeutic target for ovarian cancer

Jane Antony, Tuan Zea Tan, Zoe Kelly, Jeffrey Low, Mahesh Choolani, Chiara Recchi, Hani Gabra, Jean Paul Thiery, and Ruby Yun-Ju Huang
Sci. Signal., Oct 2016; 9: ra97;9/448/ra97

Multimodular biosensors reveal a novel platform for activation of G proteins by growth factor receptors
Krishna K. Midde, Nicolas Aznar, Melanie B. Laederich, Gary S. Ma, Maya T. Kunkel, Alexandra C. Newton, and Pradipta Ghosh
PNAS, Feb 2015; 10.1073/pnas.1420140112.