Cultrex® 10X Spheroid Formation ECM


The Cultrex® 10X Spheroid Formation ECM is a specialized extracellular matrix optimized to drive aggregation and/or spheroid formation of cells as a part of the Cultrex® 3D Culture Spheroid Cell Invasion Assay.

Available Size(s): 600μl Catalog Number: 3500-096-01 Categories: ,


The Cultrex® 10X Spheroid Formation ECM is a proprietary mixture of extracellular matrix proteins derived from murine EHS sarcoma and optimized for spheroid formation.  The diluted ECM is a fluid in culture that promotes cell-cell adhesion within a low adhesion environment.

Quality control specifications:

  • Mouse colonies are routinely screened for pathogens using mouse antibody production (MAP) testing.
  • Engelbreth-Holm-Swarm (EHS) tumor is LDEV-free.
  • PathClear® – tested negative by PCR for 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 8 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Provided in Dulbecco’s Modified Eagle’s medium (DMEM) without phenol red, with 10 µg/ml gentamicin sulfate.
  • Biological activity determined for each lot:
    • Performance tested in Cultrex® 3D Spheroid Cell Invasion Assay.


part of below kit(s)


Material Safety Data Sheet(s)

msds_3500-096-01 10X Spheroid Formation ECM