Cultrex® Organoid Harvesting Solution

Ready-to-use, non-enzymatic solution to depolymerize basement membrane matrix for harvesting organoids from culture.

Available Size(s): 100 ml Catalog Number: 3700-100-01 Categories: ,

Description

Organoid cultures exhibit cellular behaviors and morphologies similar to those seen in vivo, however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact organoids from extra-cellular proteins comprising the hydrogel. Commonly, proteases are employed to degrade these extracellular proteins, however, proteases also degrade proteins on the cell surface and protease activity may carry over into subsequent cultures or lysate preparations. Trevigen’s Cultrex® Organoid Harvesting Solution provides a non-enzymatic method for depolymerizing extracellular matrix proteins to allow for harvesting of intact organoids for passaging, cryopreservation, or biochemical analysis.

Quality control specifications:

  • Functional assay – Ten volumes of Organoid Harvesting Solution (500 μl) depolymerizes a 50 μl dome of basement membrane matrix in a 24 well plate with moderate shaking on ice for 90 minutes.

  • Sterility testing – No bacterial or fungal growth detected after incubation at 37 °C for 14 days.

  • Endotoxin concentration ≤ 20 EU/ml by LAL assay.

Storage Conditions:

Product is stable for at least 6 months from the date of receipt when stored at 2 – 8 °C. Keep sterile.

Protocol(s)

protocol_3700-100-01

Material Safety Data Sheet(s)

msds_3700-100-01 Organoid Harvesting Solution

Technical Tips(s)

Harvesting organoids for biochemical analysis

Immunofluorescence of organoids embedded in Basement Membrane Matrix

Production of R-Spondin 1 conditioned medium using Cultrex® Rspo1 cells