CultreCoat® Medium BME Cell Invasion Assay

The CultreCoat® Medium BME Cell Invasion Assays are coated with a standard density of BME for cell models that exhibit an intermediate invasive capacity. The medium amount of BME coating provides the highest sensitivity for moderately invasive cell models, giving the maximum signal to noise ratio and dynamic range. The CultreCoat® Medium BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

Available Size(s): 24 Tests, 96 tests Catalog Number: 3482-096-K Categories: , Tag:

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

The CultreCoat® Medium BME Cell Invasion Assay provides a tool for assessing cell invasion through a standardized, medium coating of reconstituted basement membrane matrix and is available in 24 and 96 well formats.

Protocol(s)

protocol_3482-024-K

protocol_3482-096-K

Material Safety Data Sheet(s)

msds_3455-096-05 10X Cell Dissociation Solution

msds_3455-096-04 25X Cell Wash Buffer

msds_4892-010-01 Calcein AM

3482-024-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

3482-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3482-096-K includes : 
Catalog Number Description Qty
3482-096-01 CultreCoat® 96 Well Medium BME Cell Invasion Chamber 1
4892-010-01 Calcein AM 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Catalog # 3482-024-K includes : 
Catalog Number Description Qty
3482-024-01 CultreCoat® 24 Well Medium BME Cell Invasion Inserts 1
4892-010-01 Calcein AM 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Cultrex® 24 Well Boyden Chamber.
Cultrex® 24 Well Boyden Chamber Diagram.
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® BME Cell Invasion Diagram.
CultreCoat® BME Invasion Assay Data.