CultreCoat® BME Cell Invasion Optimization Assay

$400.00
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The CultreCoat® Optimization System is a flexible approach that allows you to choose the desired degree of cell invasion necessary for a specific project. Included in the system are a series of Boyden chambers coated at three distinct concentrations of BME and uncoated wells to compare invasion to basal migration. Relative to uncoated wells, cell invasion decreases as the concentration of the BME coating increases. This system provides an easy approach to determine the ideal coating density that provides the maximum signal-to-noise ratio and dynamic required for your study. Once the desired concentration is determined, researchers may select the BME coated plates with the optimal coating for their study. The system is available in both 96 well and 24 well formats.

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Available Size(s): 24 Tests, 96 tests Catalog Number: 3484-096-K Categories: , Tag:

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

The CultreCoat® BME Cell Invasion Optimization Assay provides a tool for assessing cell invasion through a low, medium, and high coatings of reconstituted basement membrane matrix and is available in 24 and 96 well formats.

Protocol(s)

protocol_3484-024-K

protocol_3484-096-K

Material Safety Data Sheet(s)

msds_3455-096-05 10X Cell Dissociation Solution

msds_3455-096-04 25X Cell Wash Buffer

msds_4892-010-01 Calcein AM

3484-024-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

3484-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3484-096-K includes : 
Catalog Number Description Qty
3484-096-01 CultreCoat® 96 Well BME Cell Invasion Optimization Chamber 1
4892-010-01 Calcein AM 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Catalog # 3484-024-K includes : 
Catalog Number Description Qty
3484-024-01 CultreCoat® 24 Well BME Cell Invasion Optimization Chamber 1
4892-010-01 Calcein AM 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Cultrex® 24 Well Boyden Chamber.
Cultrex® 24 Well Boyden Chamber Diagram.
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® BME Cell Invasion Diagram.
CultreCoat® BME Invasion Assay Data.