Cultrex® Stem Cell Qualified Laminin I

$135.00

Cultrex® Stem Cell Qualified Laminin I provides a functionally defined and effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state, thereby enabling its use for growth promotion or study of stem cell differentiation.

Available Size(s): 1 ml Catalog Number: 3400-010-03 Categories: ,

Description

Cultrex® Mouse Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. This highly purified preparation of mouse Laminin I is composed of α1β1γ1 chains with a total MW of 800 KDa.

Cultrex® Stem Cell Qualified Mouse Laminin I is a purified basement membrane protein that has been developed, produced and qualified for attachment and maintenance of embryonic and inducible pluripotent stem cells.

Quality control specifications:

  • Mouse colonies are routinely screened for pathogens using mouse antibody production (MAP) testing.
  • Engelbreth-Holm-Swarm (EHS) tumor is LDEV-free.
  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • Protein concentration of 1 mg/ml.
  • No mycoplasma contamination detected by PCR.
  • Provided in Dulbecco’s Modified Eagle’s medium (DMEM) without phenol red.
  • Purity ≥90% (SDS-PAGE).
  • Biological activity determined for each lot:
    • Promotes the attachment of human iPSCs.
    • Effectively maintains human iPSCs in a pluripotent state in a feeder-free culture.

 

Protocol(s)

protocol_3400-010-03

Material Safety Data Sheet(s)

msds_3400-010-03 Stem Cell Qualified Laminin I PathClear

Human neuronal stem cells (hNSCs) cultured with Poly-Ornithine and Cultrex® Stem Cell Qualified Laminin-I and differentiated to neurons in NeuroCult™ NS-A Differentiation Medium for 18 days. Neurons were detected with anti-β tubulin III antibody (green), cell nucleus were detected with Hoechst 33342 (blue). Magnification: 20X