Cultrex® Poly-D-Lysine

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Cultrex® Poly-D-Lysine is a poly-amino acid solution that has been developed, produced and qualified as a substrate for cell adhesion.  Poly-D-Lysine contains polymers in the 70,000-150,000 Da range and is provided ready-to-use at a concentration 0.01%.

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Available Size(s): 100 ml, 2 x 100 ml Catalog Number: 3439-100-01 Category:

Description

Poly-D-Lysine, a highly positively charged synthetic amino acid chain, is commonly used as a coating agent to promote cell adhesion in culture on tissue culture-treated plastic or glass surfaces. Moreover, poly-D-Lysine is resistant to enzymatic degradation and promotes the proliferation and differentiation of a variety of neuronal cell lines. This solution contains polymers in the 70,000-150,000 Da range, and it is provided sterile filtered and ready to use at 0.01%.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/mL by LAL assay.
  • Protein concentration of 0.01%.
  • Provided in phosphate-buffered saline (PBS).
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for ability to promote attachment of rat PC-12 pheochromocytoma cells.

Protocol(s)

protocol_3439-100-01 & 3439-200-01

Material Safety Data Sheet(s)

msds_3439-100-01 Poly-D-Lysine

Catalog # 3439-200-01 includes :
Catalog Number Description Qty
3439-100-01 Poly-D-Lysine 2