Cultrex® Rat Collagen I

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Cultrex® Rat Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture. Rat Collagen I may be used as a hydrogel or thin coating for tissue culture vessels. Rat Collagen I may also be used to supplement customized coatings, hydrogels or medium formulations for cell culture.

Available Size(s): 1 ml, 20 ml Catalog Number: 3440-005-01 Category:


Cultrex® Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex® Rat Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 5 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.




Material Safety Data Sheet(s)

msds_3440-005-01 Rat Collagen-I

msds_3440-100-01 Rat Collagen-1

Glycosylation at Asn211 Regulates the Activation State of the Discoidin Domain Receptor 1 (DDR1)
Fu, H.L., Valiathan, R.R., Payne, L., Kumarasiri, M., Mahasenan, K.V., Mobashery, S., Huang, P. and Fridman, R.
J. Biol. Chem., Mar 2014; 289: 9275 – 9287.

RPTPα controls epithelial adherens junctions, linking E-cadherin engagement to c-Src-mediated phosphorylation of cortactin
Marta Truffi, Véronique Dubreuil, Xuan Liang, Nathalie Vacaresse, Fabienne Nigon, Siew Ping Han, Alpha S. Yap, Guillermo A. Gomez, and Jan Sap
J. Cell Sci., Jun 2014; 127: 2420 – 2432.

Biphasic Modulation of Paracellular Claudin-5 Expression in Mouse Brain Endothelial Cells Is Mediated through the Phosphoinositide-3-Kinase/AKT Pathway
Ryan B. Camire, Holly J. Beaulac, Stephanie A. Brule, Annie I. McGregor, Emily E. Lauria, and Colin L. Willis
J. Pharmacol. Exp. Ther., Oct 2014; 351: 654 – 662.

DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity
Qian Zhang, Christopher G. Dove, Jyh Liang Hor, Heardley M. Murdock, Dara M. Strauss-Albee, Jordan A. Garcia, Judith N. Mandl, Rachael A. Grodick, Huie Jing, Devon B. Chandler-Brown, Timothy E. Lenardo, Greg Crawford, Helen F. Matthews, Alexandra F. Freeman, Richard J. Cornall, Ronald N. Germain, Scott N. Mueller, and Helen C. Su
J. Exp. Med., Nov 2014; 10.1084/jem.20141307.

“Maspin Expression in Prostate Tumor Cells Averts Stemness and
Stratifies Drug Sensitivity”

“M. Margarida Bernardo, Alexander Kaplun, Sijana H. Dzinic, Xiaohua Li, Jonathan Irish, Adelina Mujagic,
Benjamin Jakupovic, Jessica B. Back, Eric Van Buren, Xiang Han, Ivory Dean, Yong Q. Chen, Elisabeth
Heath, Wael Sakr, and Shijie Sheng”
Cancer Res., Sep 2015; 75: 3970 – 3979.

Mesencephalic Astrocyte–Derived Neurotrophic Factor as a Urine Biomarker for Endoplasmic Reticulum Stress–Related Kidney Diseases

Yeawon Kim, Heedoo Lee, Scott R. Manson, Maria Lindahl, Bradley Evans, Jeffrey H. Miner, Fumihiko Urano, and Ying Maggie Chen
J. Am. Soc. Nephrol., Mar 2016; 10.1681/ASN.2014100986