Cultrex® Rat Collagen I

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Cultrex® Rat Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture. Rat Collagen I may be used as a hydrogel or thin coating for tissue culture vessels. Rat Collagen I may also be used to supplement customized coatings, hydrogels or medium formulations for cell culture.

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Available Size(s): 1 ml, 20 ml Catalog Number: 3440-005-01 Category:

Description

Cultrex® Rat Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex® Rat Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 5 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.

Protocol(s)

protocol_3440-005-01

protocol_3440-100-01

Material Safety Data Sheet(s)

msds_3440-005-01 Rat Collagen-I

msds_3440-100-01 Rat Collagen-1

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