Cultrex® Bovine Collagen I

Cultrex® Bovine Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture. Bovine Collagen I may be used as a hydrogel or thin coating for tissue culture vessels. Bovine Collagen I may also be used to supplement customized coatings, hydrogels or medium formulations for cell culture.

Available Size(s): 1 ml, 50 mg Catalog Number: 3442-005-01 Category:


Cultrex® Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex® Bovine Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 5 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.




Material Safety Data Sheet(s)

msds_3442-005-01 Bovine Collagen-1

msds_3442-050-01 Bovine Collagen-I

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Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling
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PNAS, Jun 2014; 10.1073/pnas.1405388111.

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System
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J. Immunol., Feb 2016; 196: 1558 – 1567.