Cultrex® Bovine Collagen I

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Cultrex® Bovine Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture. Bovine Collagen I may be used as a hydrogel or thin coating for tissue culture vessels. Bovine Collagen I may also be used to supplement customized coatings, hydrogels or medium formulations for cell culture.

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Available Size(s): 1 ml, 50 mg Catalog Number: 3442-005-01 Category:

Description

Cultrex® Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL.

Cultrex® Bovine Collagen I is a purified stromal ECM protein that has been developed, produced and qualified for general cell culture applications.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 5 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.

Protocol(s)

protocol_3442-005-01

protocol_3442-050-01

Material Safety Data Sheet(s)

msds_3442-005-01 Bovine Collagen-1

msds_3442-050-01 Bovine Collagen-I

Functionally distinct PI 3-kinase pathways regulate myelination in the peripheral nervous system
Heller, B.A., Ghidinelli, M., Voelkl, J., Einheber, S., Smith, R., Grund, E., Morahan, G., Chandler, D., Kalaydjieva, L., Giancotti, F., King, R.H., Fejes-Toth, A.N., Fejes-Toth, G., Feltri, M.L., Lang, F. and Salzer, J.L.
J. Cell Biol., Mar 2014; 204: 1219 – 1236.
http://jcb.rupress.org/cgi/content/abstract/204/7/1219

Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling
Ruei-Zeng Lin, Rafael Moreno-Luna, Dan Li, Shou-Ching Jaminet, Arin K. Greene, and Juan M. Melero-Martin
PNAS, Jun 2014; 10.1073/pnas.1405388111.
http://www.pnas.org/cgi/content/abstract/1405388111v1

Pivotal Role for CD16+ Monocytes in Immune Surveillance of the Central Nervous System
Anne Waschbisch, Sina Schröder, Dana Schraudner, Laura Sammet, Babette Weksler, Arthur Melms, Sabine Pfeifenbring, Christine Stadelmann, Stefan Schwab, and Ralf A. Linker
J. Immunol., Feb 2016; 196: 1558 – 1567.
http://www.jimmunol.org/cgi/content/abstract/196/4/1558