Cultrex® Basement Membrane Matrix, Type 2

Cultrex® BME Type 2 is a Basement Membrane Matrix that has been developed, produced and qualified specifically for robust organoid cultures. BME Type 2 is an extracellular matrix hydrogel that has been used extensive for the establishment and expansion of organoids. BME Type 2 mimics the in vivo microenvironment to improve take rate and growth of organoid progenitor cells.

Available Size(s): 1 ml, 2 x 5 ml, 5 ml Catalog Number: 3532-001-02 Categories: , , Tags: ,

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex® BME Type 2 is a Basement Membrane Matrix that has been developed, produced and qualified specifically for robust organoid cultures.

Quality control specifications:

  • Mouse colonies are routinely screened for pathogens using mouse antibody production (MAP) testing.
  • Engelbreth-Holm-Swarm (EHS) tumor is LDEV-free.
  • PathClear® – tested negative by PCR for 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 8 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • Protein concentration of 12 – 18 mg/ml.
  • No mycoplasma contamination detected by PCR.
  • Provided in Dulbecco’s Modified Eagle’s medium (DMEM) without phenol red, with 10 µg/ml gentamicin sulfate.
  • Gel stability tested for a period of 14 days at 37°C.
  • Biological activity determined for each lot:
    • Tube formation assay – BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.
    • Organoid culture: Basement Membrane Extract promotes growth and expansion of mouse small intestine organoid progenitor cells.

Protocol(s)

protocol_3532-001-02

protocol_3532-005-02

protocol_3532-010-02

Material Safety Data Sheet(s)

3532-001-02 msds_3532-001-02 Cultrex Basement Membrane Extract Type 2 PathClear

3532-005-02 msds_3532-005-02 Cultrex Basement Membrane Extract Type 2 PathClear

3532-005-02 msds_3532-010-02 Cultrex Basement Membrane Extract, Type 2, PathClear

Technical Tips(s)

Harvesting organoids for biochemical analysis

Immunofluorescence of organoids embedded in Basement Membrane Matrix

Production of R-Spondin 1 conditioned medium using Cultrex® Rspo1 cells

Catalog # 3532-010-01 includes : 
Catalog Number Description Qty
3532-005-01 Cultrex Basement Membrane Extract Type 2, PathClear® 2

 

Cultrex® BME Endothelial Tube Formation.

“Basement Membrane-Based Glucose Sensor Coatings Enhance
Continuous Glucose Monitoring in Vivo”

Ulrike Klueh, Yi Qiao, Caroline Czajkowski, Izabela Ludzinska, Omar Antar, and Donald L. Kreutzer
Journal of Diabetes Science and Technology, Sep 2015; 9: 957 – 965.
http://dst.sagepub.com/cgi/content/abstract/9/5/957