Cultrex® Reduced Growth Factor Basement Membrane Matrix

Cultrex® RGF BME is a Reduced Growth Factor Basement Membrane Matrix that has been developed, produced and qualified for general cell culture applications. RGF BME is an extracellular matrix hydrogel that has been used extensively to study cell attachment, proliferation, and invasion.

Available Size(s): 1 ml, 2 x 5 ml, 5 ml Catalog Number: 3433-001-01 Category: Tags: ,

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex® RGF BME is a Basement Membrane Matrix that has been developed, produced and qualified for general cell culture applications. Cultrex® RGF BME is processed to reduce matrix-associated growth factors and provide a more defined model system.

Quality control specifications:

  • Mouse colonies are routinely screened for pathogens using mouse antibody production (MAP) testing.
  • Engelbreth-Holm-Swarm (EHS) tumor is LDEV-free.
  • PathClear® – tested negative by PCR for 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.
  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 8 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • Protein concentration of 12 – 18 mg/ml.
  • No mycoplasma contamination detected by PCR.
  • Provided in Dulbecco’s Modified Eagle’s medium (DMEM) without phenol red, with 10 µg/ml gentamicin sulfate.
  • Gel stability tested for a period of 14 days at 37°C.
  • Biological activity determined for each lot:
    • Tube formation assay – BME promotes formation of capillary-like structures by human (HBMVEC; HUVEC) or mouse (SVEC4-10) endothelial cells.

Protocol(s)

protocol_3433-001-01

protocol_3433-005-01

protocol_3433-010-01

Material Safety Data Sheet(s)

msds_3433-001-01 Cultrex Reduced Growth Factor Basement Membrane ExtractPathClear

msds_3433-005-01 Reduced Growth Factor Basement Membrane Extract PathClear

msds_3433-010-01 Cultrex Reduced Growth Factor Basement Membrane Extract PathClear

Catalog # 3433-010-01 includes :
Catalog Number Description Qty
3433-005-01 Reduced Growth Factor Basement Membrane Extract, PathClear® 2
Cultrex® BME Endothelial Tube Formation.

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http://cancerres.aacrjournals.org/cgi/content/abstract/74/15/4053

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Paulo S. Caceres, Mariela Mendez, and Pablo A. Ortiz
J. Biol. Chem., Aug 2014; 289: 23951 – 23962.
http://www.jbc.org/cgi/content/abstract/289/34/23951

The M33 G Protein-Coupled Receptor Encoded by Murine Cytomegalovirus Is Dispensable for Hematogenous Dissemination but Is Required for Growth within the Salivary Gland
Fabiola M. Bittencourt, Shu-En Wu, James P. Bridges, and William E. Miller
J. Virol., Oct 2014; 88: 11811 – 11824.
http://jvi.asm.org/cgi/content/abstract/88/20/11811

Immunoevasive Pericytes From Human Pluripotent Stem Cells Preferentially Modulate Induction of Allogeneic Regulatory T Cells
Hagit Domev, Irina Milkov, Joseph Itskovitz-Eldor, and Ayelet Dar
Stem Cells Trans Med, Oct 2014; 3: 1169 – 1181.
http://stemcellstm.alphamedpress.org/cgi/content/abstract/3/10/1169

PTEN inhibits PREX2-catalyzed activation of RAC1 to restrain tumor cell invasion
Sarah M. Mense, Douglas Barrows, Cindy Hodakoski, Nicole Steinbach, David Schoenfeld, William Su, Benjamin D. Hopkins, Tao Su, Barry Fine, Hanina Hibshoosh, and Ramon Parsons
Sci. Signal., Mar 2015; 8: ra32.
http://stke.sciencemag.org/cgi/content/abstract/sigtrans;8/370/ra32

“Effects of ulipristal acetate on human embryo attachment
and endometrial cell gene expression in an in vitro
co-culture system”

“C. Berger, N.R. Boggavarapu, J. Menezes, P.G.L. Lalitkumar, and K. Gemzell-Danielsson”
Hum. Reprod., Apr 2015; 30: 800 – 811.
http://humrep.oxfordjournals.org/cgi/content/abstract/30/4/800

“The Hippo Pathway Effector YAP Regulates Motility,
Invasion, and Castration-Resistant Growth of Prostate
Cancer Cells”

“Lin Zhang, Shuping Yang, Xingcheng Chen, Seth Stauffer, Fang Yu, Subodh M. Lele,
Kai Fu, Kaustubh Datta, Nicholas Palermo, Yuanhong Chen, and Jixin Dong”
Mol. Cell. Biol., Apr 2015; 35: 1350 – 1362.
http://mcb.asm.org/cgi/content/abstract/35/8/1350

Conditioned Medium From Human Amniotic Mesenchymal Stromal Cells Limits Infarct Size and Enhances Angiogenesis
“Patrizia Danieli, Giuseppe Malpasso, Maria Chiara Ciuffreda, Elisabetta Cervio, Laura Calvillo, Francesco Copes, Federica Pisano, Manuela Mura, Lennaert Kleijn,
Rudolf A. de Boer, Gianluca Viarengo, Vittorio Rosti, Arsenio Spinillo, Marianna Roccio, and Massimiliano Gnecchi”
Stem Cells Trans Med, May 2015; 4: 448 – 458.
http://stemcellstm.alphamedpress.org/cgi/content/abstract/4/5/448

Loss of PPAR in endothelial cells leads to impaired angiogenesis
Sanna Vattulainen-Collanus, Oyediran Akinrinade, Molong Li, Minna Koskenvuo, Caiyun Grace Li, Shailaja P. Rao, Vinicio de Jesus Perez, Ke Yuan, Hirofumi Sawada, Juha W. Koskenvuo, Cristina Alvira, Marlene Rabinovitch, and Tero-Pekka Alastalo
J. Cell Sci., Feb 2016; 129: 693 – 705.
http://jcs.biologists.org/cgi/content/abstract/129/4/693

“Fluorescent Image–Guided Surgery with an Anti-Prostate Stem Cell
Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real Time”

Geoffrey A. Sonn, Andrew S. Behesnilian, Ziyue Karen Jiang, Kirstin A. Zettlitz, Eric J. Lepin, Laurent A. Bentolila, Scott M. Knowles, Daniel Lawrence, Anna M. Wu, and Robert E. Reiter
Clin. Cancer Res., Mar 2016; 22: 1403 – 1412.
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/6/1403

“Grainyhead-like 2 downstream targets act to suppress epithelialto-
mesenchymal transition during neural tube closure”

Heather J. Ray and Lee A. Niswander
Development, Apr 2016; 143: 1192 – 1204.
http://dev.biologists.org/cgi/content/abstract/143/7/1192

“Src as a Therapeutic Target in Biliary Tract Cancer”

“Ah-Rong Nam, Ji-Won Kim, Ji Eun Park, Ju-Hee Bang, Mei Hua Jin, Kyung-Hun Lee, Tae-Yong Kim,
Sae-Won Han, Seock-Ah Im, Tae-You Kim, Do-Youn Oh, and Yung-Jue Bang”
Mol. Cancer Ther., Jul 2016; 15: 1515 – 1524.
http://mct.aacrjournals.org/cgi/content/abstract/15/7/1515

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J. Biol. Chem., Oct 2016; 291: 22063 – 22073
http://www.jbc.org/cgi/content/abstract/291/42/22063

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“Roberto Rangel, Song-Choon Lee, Kenneth Hon-Kim Ban, Liliana Guzman-Rojas, Michael B. Mann, Justin
Y. Newberg, Takahiro Kodama, Leslie A. McNoe, Luxmanan Selvanesan, Jerrold M. Ward, Alistair G. Rust,
Kuan-Yew Chin, Michael A. Black, Nancy A. Jenkins, and Neal G. Copeland”
PNAS, Nov 2016; 113: E7749 – E7758.
http://www.pnas.org/cgi/content/abstract/113/48/E7749

“Preclinical Efficacy of the Auristatin-Based Antibody–Drug Conjugate
BAY 1187982 for the Treatment of FGFR2-Positive Solid Tumors”

“Anette Sommer, Charlotte Kopitz, Christoph A. Schatz, Carl F. Nising, Christoph Mahlert, Hans-Georg
Lerchen, Beatrix Stelte-Ludwig, Stefanie Hammer, Simone Greven, Joachim Schuhmacher, Manuela
Braun, Ruprecht Zierz, Sabine Wittemer-Rump, Axel Harrenga, Frank Dittmer, Frank Reetz, Heiner
Apeler, Rolf Jautelat, Hung Huynh, Karl Ziegelbauer, and Bertolt Kreft”
Cancer Res., Nov 2016; 76: 6331 – 6339
http://cancerres.aacrjournals.org/cgi/content/abstract/76/21/6331

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Vishnu Priyanka Reddy Chichili, J. Sivaraman, Thomas Putti, Aye Aye Thike, Puay Hoon Tan, Marius
Sudol, David M. Virshup, Siew Wee Chan, Wanjin Hong, and Yoon Pin Lim”
Cancer Res., Nov 2016; 76: 6278 – 6289.
http://cancerres.aacrjournals.org/cgi/content/abstract/76/21/6278

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Spinella, F., Caprara, V., Cianfrocca, R., Rosano, L., Di Castro, V., Garrafa, E., Natali, P.G., andBagnato, A.The interplay between hypoxia, endothelial and melanoma cells regulates vascularization and cell motility through endothelin-1 and vascular endothelial growth factor41687
Carcinogenesis, Feb 2014; 10.1093/carcin/bgu018.
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu018v2

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Trivisonno, A., Di Rocco, G., Cannistra, C., Finocchi, V., Torres Farr, S., Monti, M. and Toietta, G.Harvest of Superficial Layers of Fat With a Microcannula and Isolation of Adipose Tissue-Derived Stromal and Vascular CellsJournal Article
Aesthetic Surgery Journal, May 2014; 34: 601 – 613.
http://aes.sagepub.com/cgi/content/abstract/34/4/601