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Cultrex® 3D Culture Matrix Rat Collagen I

$165.00

Cultrex® 3D Culture Matrix® Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3D culture studies. 3D Culture Matrix® Collagen I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro. 3D Culture Matrix® Collagen I may also be used to supplement customized hydrogel or medium formulations for cell culture.

Available Size(s): 20 ml Catalog Number: 3447-020-01 Categories: ,

Product Description

Cultrex® 3D Culture Matrix® Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.

Cultrex® 3D Culture Matrix® Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3D culture studies.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 5 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Biological activity determined for each lot:
    • 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells.
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.

Protocol(s)

protocol_3447-020-01

Material Safety Data Sheet(s)

msds_3447-020-01 3-D Culture Matrix Collagen I Rat Tail

Cultrex® 3D Culture.

“Organotypic Culture of Untransformed and Tumorigenic Primary Mammary Epithelial Cells”
Martin Jechlinger
Cold Spring Harb Protoc, May 2015; 2015: pdb.prot078295
http://cshprotocols.cshlp.org/cgi/content/abstract/2015/5/pdb.prot078295