Description
Cultrex® 3D Culture Matrix® Collagen I is purified from rat tail tendons and is provided at a high concentration that is capable of polymerizing to form a hydrogel. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of approximately 5 mg/mL. This material is then incorporated in a 3-D culture to validate efficacy.
Cultrex® 3D Culture Matrix® Collagen I is a purified stromal ECM protein that has been developed, produced and qualified specifically for use in 3D culture studies.
Quality control specifications:
- No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
- Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
- No mycoplasma contamination detected by PCR.
- Protein concentration of 5 mg/ml.
- Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
- Biological activity determined for each lot:
- 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells.
- Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
