Cultrex® Cell Migration Assay

Cultrex® Cell Migration Assays were originally created in an effort to accelerate the screening process for compounds that influence chemotaxis, which is a fundamental component of angiogenesis in embryonic development, immune responses, and wound healing. These assays offer a flexible, standardized, multi-well format for the quantification of the degree to which cells migrate in vitro in response to chemoattractants in the presence of inhibitors or stimulants. The Cultrex® Cell Migration Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

Available Size(s): 24 Tests, 96 tests Catalog Number: 3465-024-K Category:

Description

The Cultrex® Cell Migration Assays utilizes a simplified Boyden chamber design with an 8 micron polyethylene terephthalate (PET) membrane.  Cells attach to and migrate through the membrane in response to chemokines and growth factors.  The porosity of this membrane is most appropriate for adherent epithelial or endothelial cells based on cell size. These assays are available in 24 and 96 well formats.

The CultreCoat® Cell Migration Assays provides a tool for assessing chemotactic response through an uncoated membrane and is available in 24 and 96 well formats.

Protocol(s)

protocol_3465-024-K

protocol_3465-096-K

Material Safety Data Sheet(s)

msds_3455-096-05 10X Cell Dissociation Solution

msds_3455-096-04 25X Cell Wash Buffer

msds_4892-010-01 Calcein AM

3465-024-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

3465-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3465-096-K includes : 
Catalog Number Description Qty
3455-024-01 Cell Migration Chamber 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
4892-010-01 Calcein AM 1
Catalog # 3465-024-K includes :
Catalog Number Description Qty
3455-024-01 Cell Migration Inserts 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
4892-010-01 Calcein AM 1
Cultrex® 24 Well Boyden Chamber.
Cultrex® 24 Well Boyden Chamber Diagram.
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® Cell Migration Diagram.
Cultrex® Cell Migration and Invasion Data.

Reprogramming of Monocytes by GM-CSF Contributes to Regulatory Immune Functions during Intestinal Inflammation
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“Fenretinide Perturbs Focal Adhesion Kinase in
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Fenretinide’s Chemopreventive Mechanisms Include ECM
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Tumor-Induced Pressure in the Bone Microenvironment Causes Osteocytes to Promote the Growth of Prostate Cancer Bone Metastases
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http://cancerres.aacrjournals.org/cgi/content/abstract/75/11/2151

A Novel Peroxisome Proliferator-activated Receptor (PPAR){gamma} Agonist 2-Hydroxyethyl 5-chloro-4,5-didehydrojasmonate Exerts Anti-Inflammatory Effects in Colitis
Jieun Choo, Yunna Lee, Xin-jia Yan, Tae Hwan Noh, Seong Jin Kim, Sujin Son, Charalabos Pothoulakis, Hyung Ryong Moon, Jee H. Jung, and Eunok Im
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SIRT6 deacetylates PKM2 to suppress its nuclear localization and oncogenic functions
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PNAS, Feb 2016; 113: E538 – E547.
http://www.pnas.org/cgi/content/abstract/113/5/E538

Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli
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Infect. Immun., Apr 2016; 84: 976 – 988.
http://iai.asm.org/cgi/content/abstract/84/4/976

“Musashi-2 (MSI2) supports TGF-β signaling and inhibits claudins to
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