Cultrex® 5X Collagen I Solution

$110.00

Cultrex® 5X Collagen I solution has been developed, manufactured and qualified as a coating for the Cultrex® Collagen I Cell Invasion Assays.

Available Size(s): 1 ml Catalog Number: 3457-096-02 Category:

Description

Cultrex® Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of 1 mg/mL.

Cultrex® 5X Collagen I Solution is a purified stromal ECM protein that has been developed, produced and qualified for the Cultrex® Collagen I Cell Invasion Assays.

Quality control specifications:

  • No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
  • Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
  • No mycoplasma contamination detected by PCR.
  • Protein concentration of 1 mg/ml.
  • Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
  • Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
  • Biological activity determined for each lot:
    • Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
    • Performance tested in Cultrex® Collagen I Cell Invasion Assay.

Protocol(s)

Part of below Kit(s)

protocol_3457-024-K

protocol_3457-096-K

Material Safety Data Sheet(s)

msds_3457-096-02 5X Collagen I Solution