Cultrex® Bovine Collagen I is purified from fetal bovine extensor tendons and is pepsin-treated to remove telopeptides. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37̊ C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of 1 mg/mL.
Cultrex® 5X Collagen I Solution is a purified stromal ECM protein that has been developed, produced and qualified for the Cultrex® Collagen I Cell Invasion Assays.
Quality control specifications:
- No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP sterility testing guidelines.
- Endotoxin concentration ≤ 20 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.
- No mycoplasma contamination detected by PCR.
- Protein concentration of 1 mg/ml.
- Provided in 20 mM Acetic Acid, pH 3.4 – 3.6.
- Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH.
- Biological activity determined for each lot:
- Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
- Performance tested in Cultrex® Collagen I Cell Invasion Assay.