Cultrex® BME Cell Invasion Assay

$350.00 – $370.00
Select an Option to See Pricing

The Cultrex® BME Cell Invasion Assays provide systems where the researcher can determine the optimal BME coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through BME may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex® BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

Clear
Available Size(s): 24 Tests, 96 tests Catalog Number: 3455-024-K Category:

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

The Cultrex® BME Cell Invasion Assay provides a tool for assessing cell invasion through a reconstituted basement membrane and is available in 24 and 96 well formats.

Protocol(s)

protocol_3455-024-K

protocol_3455-096-K

Material Safety Data Sheet(s)

msds_3455-096-02 5X BME Solution

msds_3455-096-05 10X Cell Dissociation Solution

msds_3455-096-04 25X Cell Wash Buffer

msds_3455-096-03 10X Coating Buffer

msds_4892-010-01 Calcein AM

3455-024-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

3455-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3455-024-K includes :
Catalog Number Description Qty
3455-096-02 5X BME Coating Solution 1
3455-096-03 10X Coating Buffer 1
4892-010-01 Calcein AM 1
3455-024-01 Cell Invasion Inserts 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Catalog # 3455-096-K includes :
Catalog Number Description Qty
3455-096-02 5X BME Coating Solution 1
3455-096-03 10X Coating Buffer 1
4892-010-01 Calcein AM 1
3455-096-01 Cell Invasion Chamber 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Cultrex® 24 Well Boyden Chamber.
Cultrex® 24 Well Boyden Chamber Diagram.
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® BME Cell Invasion Diagram.
Cultrex® Cell Migration and Invasion Data.

Hypermethylation of the GABRE\textasciitildemiR-452\textasciitildemiR-224 Promoter in Prostate Cancer Predicts Biochemical Recurrence after Radical Prostatectomy,
Helle Kristensen, Christa Haldrup, Siri Strand, Kamilla Mundbjerg, Martin M. Mortensen, Kasper Thorsen, Marie Stampe Ostenfeld, Peter J. Wild, Christian Arsov, Wolfgang Goering, Tapio Visakorpi, Lars Egevad, Johan Lindberg, Henrik Grönberg, Søren Høyer, Michael Borre, Torben F. Ørntoft, and Karina D. Sørensen
Clin. Cancer Res., Apr 2014; 20: 2169 – 2181.
http://clincancerres.aacrjournals.org/cgi/content/abstract/20/8/2169

1α,25-Dihydroxyvitamin D3 Inhibits Esophageal Squamous Cell Carcinoma Progression by Reducing IL6 Signaling
Ping-Tsung Chen, Ching-Chuan Hsieh, Chun-Te Wu, Tzu-Chen Yen, Paul-Yang Lin, Wen-Cheng Chen, and Miao-Fen Chen
Mol. Cancer Ther., Jun 2015; 14: 1365 – 1375.
http://mct.aacrjournals.org/cgi/content/abstract/14/6/1365

Reciprocal regulation of amino acid import and epigenetic state through Lat1 and EZH2
Stephen G Dann, Michael Ryskin, Anthony M Barsotti, Jonathon Golas, Celine Shi, Miriam Miranda, Christine Hosselet, Luanna Lemon, Judy Lucas, Maha Karnoub, Fang Wang, Jeremy S Myers, Scott J Garza, Maximillian T Follettie, Kenneth G Geles, Anke Klippel, Robert A Rollins, and Valeria R Fantin
EMBO J., Jul 2015; 34: 1773 – 1785.
http://EMBOJ.embopress.org/cgi/content/abstract/34/13/1773

“Semaphorin 3D autocrine signaling mediates the metastatic role of
annexin A2 in pancreatic cancer”
“Kelly Foley, Agnieszka A. Rucki, Qian Xiao, Donger Zhou, Ashley Leubner, Guanglan Mo, Jennifer
Kleponis, Annie A. Wu, Rajni Sharma, Qingguang Jiang, Robert A. Anders, Christine A. Iacobuzio-
Donahue, Katherine A. Hajjar, Anirban Maitra, Elizabeth M. Jaffee, and Lei Zheng”
Sci. Signal., Aug 2015; 8: ra77.
http://stke.sciencemag.org/cgi/content/abstract/sigtrans;8/388/ra77

Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels
Ken Fujimura, Sunkyu Choi, Meghan Wyse, Jan Strnadel, Tracy Wright, and Richard Klemke
J. Biol. Chem., Dec 2015; 290: 29907 – 29919.
http://www.jbc.org/cgi/content/abstract/290/50/29907

“Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase
Isoform Expression and Targeting to the Plasma Membrane and
Endosomes”
Gina A. Smith, Gareth J. Howell, Clair Phillips, Stephen P. Muench, Sreenivasan Ponnambalam, and Michael A. Harrison
J. Biol. Chem., Apr 2016; 291: 8500 – 8515.
http://www.jbc.org/cgi/content/abstract/291/16/8500

The MCT4 Gene: A Novel, Potential Target for Therapy of Advanced Prostate Cancer
“Stephen Yiu Chuen Choi, Hui Xue, Rebecca Wu, Ladan Fazli, Dong Lin, Colin C. Collins, Martin E. Gleave,
Peter W. Gout, and Yuzhuo Wang”
Clin. Cancer Res., Jun 2016; 22: 2721 – 2733.
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/11/2721

Related products