Cultrex® BME Cell Invasion Assay

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The Cultrex® BME Cell Invasion Assays provide systems where the researcher can determine the optimal BME coating for their research. Since different cell lines and different treatments can result in a wide range of invasive potentials, the permissiveness of each cell line to invade through BME may be optimized to fit each experiment by adjusting the coating concentration. The Cultrex® BME Cell Invasion Assays are provided as either a single 96 well plate providing capacity for large screening experiments or as 24 individual inserts providing flexibility for smaller studies.

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Available Size(s): 24 Tests, 96 tests Catalog Number: 3455-024-K Category:

Description

Cultrex® Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

The Cultrex® BME Cell Invasion Assay provides a tool for assessing cell invasion through a reconstituted basement membrane and is available in 24 and 96 well formats.

Protocol(s)

protocol_3455-024-K

protocol_3455-096-K

Material Safety Data Sheet(s)

msds_3455-096-02 5X BME Solution

msds_3455-096-05 10X Cell Dissociation Solution

msds_3455-096-04 25X Cell Wash Buffer

msds_3455-096-03 10X Coating Buffer

msds_4892-010-01 Calcein AM

3455-024-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

3455-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3455-024-K includes :
Catalog Number Description Qty
3455-096-02 5X BME Coating Solution 1
3455-096-03 10X Coating Buffer 1
4892-010-01 Calcein AM 1
3455-024-01 Cell Invasion Inserts 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Catalog # 3455-096-K includes :
Catalog Number Description Qty
3455-096-02 5X BME Coating Solution 1
3455-096-03 10X Coating Buffer 1
4892-010-01 Calcein AM 1
3455-096-01 Cell Invasion Chamber 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Cultrex® 24 Well Boyden Chamber.
Cultrex® 24 Well Boyden Chamber Diagram.
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® BME Cell Invasion Diagram.
Cultrex® Cell Migration and Invasion Data.

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