Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

The Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit allows for the detection of inducers and inhibitors of endothelial cell invasion through a basement membrane. This technology utilizes the autonomous endothelial cell response of invasion when seeded on a basement membrane matrix in the presence of the appropriate growth factors. The Cultrex® In Vitro Angiogenesis Endothelial Cell Invasion Kit is designed to interrogate endothelial invasion in response to compounds and soluble factors.

Available Size(s): 96 tests Catalog Number: 3471-096-K Category:

Description

The Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell digestion of and invasion through a basement membrane extract (BME) layer. This kit offers a flexible, standardized, 96 well high-throughput format for quantifying endothelial invasion, in vitro, in response to chemoattractants and/or inhibiting compounds. This assay employs a simplified Boyden chamber-like design with an 8 micron polyethylene terephthalate (PET) membrane. Sulforaphane [1-isothiocyanato-(4R)-methylsulfinyl)-butane], a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of in vitro endothelial cell invasion through Cultrex® BME. Detection of cell invasion is quantified using Calcein-AM, which is internalized by the cells, and converted to the acetomethylester (AM) moiety by intracellular esterases. Free Calcein fluoresces brightly, and this fluorescence may be used to quantify the number of cells that have invaded using a fluorescence plate reader.

Protocol(s)

protocol_3471-096-K

Material Safety Data Sheet(s)

msds_3455-096-02 5X BME Solution

msds_3455-096-03 10X Coating Buffer

msds_3455-096-04 25X Cell Wash Buffer

msds_3455-096-05 10X Cell Dissociation Solution

msds_4892-010-01 Calcein AM

msds_3470-096-02 Sulforaphane 10mM

3455-096-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 3471-096-K includes :
Catalog Number Description Qty
3455-096-02 5X BME Coating Solution 1
3470-096-02 Sulforaphane 1
4892-010-01 Calcein AM 1
3455-096-01 Cell Invasion Chamber 1
3455-096-03 10X Coating Buffer 1
3455-096-04 25X Cell Wash Buffer 1
3455-096-05 10X Cell Dissociation Solution 1
Cultrex® 96 Well Boyden Chamber.
Cultrex® 96 Well Boyden Chamber Diagram.
Cultrex® BME Cell Invasion Diagram.
Cultrex® Endothelial Cell Invasion Data.