Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit

The Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit allows for the detection of inducers and inhibitors of endothelial cell tube formation. This technology utilizes the autonomous endothelial cell response of self organization into microvessels, also known as tubes, when seeded on a basement membrane matrix in the presence of the appropriate growth factors. The Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit is designed to interrogate endothelial assembly in response to compounds and soluble factors.

Available Size(s): 96 Wells Catalog Number: 3470-096-K Category:

Description

The Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit was developed for the evaluation of inducers and inhibitors of endothelial cell tube formation.  During the course of the assay, endothelial cells are seeded on a thick gel of Basement Membrane Extract in the presence of angiogenesis modulating agents.  If the medium is permissive to the angiogenic response, cells will self assemble into tube networks within hours; if there are inhibitors in the medium, tube network formation will be incomplete or nonexistent. Sulforaphane [1- isothiocyanato-(4R)- methylsulfinyl)-butane], found in broccoli and other cruciferous vegetables, is a naturally occurring cancer chemopreventive agent, and is provided as a control inhibitor to visualize this process.  Endothelial cells may be fluorescently labeled using Calcein AM, and images captured using a microscope camera may be analyze to quantify the angiogenic response. Cultrex® In Vitro Angiogenesis Assay Kit provides a cost-effective method for investigation of prospective angiogenesis inhibitors in a 96 well plate format.

Protocol(s)

protocol_3470-096-K

Material Safety Data Sheet(s)

msds_3433-005-01 Reduced Growth Factor Basement Membrane Extract PathClear

msds_3470-096-01 Cell Staining Solution

msds_4892-010-01 Calcein AM

msds_3470-096-02 Sulforaphane 10mM

Catalog # 3470-096-K includes :
Catalog Number Description Qty
3470-096-01 Cell Staining Solution 1
3433-005-01 Cultrex® BME, reduced growth factor without phenol red 1
3470-096-02 Sulforaphane 1
4892-010-01 Calcein AM 1
Cultrex® Endothelial Tube Formation Diagram.
Cultrex® Endothelial Tube Formation Over Time.
Cultrex® Endothelial Tube Formation Inhibition.

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Tram Thu Vuong, Trine M. Reine, Amanda Sudworth, Trond G. Jenssen, and Svein O. Kolset
Journal of Histochemistry & Cytochemistry, Feb 2015; 10.1369/0022155415568995
http://jhc.sagepub.com/cgi/content/abstract/0022155415568995v2

Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis
Zheng Chen, Shoumin Zhu, Jun Hong, Mohammed Soutto, DunFa Peng, Abbes Belkhiri, Zekuan Xu, and Wael El-Rifai
Gut, Mar 2015; 10.1136/gutjnl-2014-308416.
http://gut.bmj.com/cgi/content/abstract/gutjnl-2014-308416v1

“The Role of Angiogenesis in the Persistence of Chemoresistance in
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“Osama Nusrat, Jimmy Belotte, Nicole M. Fletcher, Ira Memaj, Mohammed G. Saed, Michael P. Diamond,
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http://rsx.sagepub.com/cgi/content/abstract/23/11/1484