Cultrex® 3D Culture Cell Harvest Buffers

The Cultrex® 3D Culture Cell Harvest Buffers are an optimized and standardized solution for the isolation of intact 3D structures from Basement Membrane Extract (BME) Matrix or Laminin I hydrogels as a part of the Cultrex® 3D Culture Cell Harvesting Kit.

Available Size(s): 100 ml each Catalog Number: 3448-020-CH Category:

Description

3D Cultures exhibit cellular behaviors and morphologies similar to those seen in vivo; however, the adaptation of these models for studying biochemical processes has been impeded by the challenge of separating intact cells from extracellular matrix hydrogels.  Commonly, proteases are employed to degrade these extracellular proteins; however, proteases also degrade proteins on the cell surface and may carry over into lysate preparations. Non-enzymatic methodologies have also been described for depolymerizing extracellular matrix proteins, although the implementation of these protocols remains problematic for some researchers. The Cultrex® 3D Culture Cell Harvest Buffers depolymerize BME and Laminin I hydrogels, non-enzymatically, providing an optimized and standardized solution for the isolation of 3D structures.

Protocol(s)

Part of below Kit(s)

protocol_3448-020-K

Material Safety Data Sheet(s)

msds_3448-020-01 3-D Culture 10X Cell Harvesting Buffer

msds_3448-020-02 3-D Culture 10X Cell Wash Buffer

3D Culture Cell Harvest Buffers 3448-020-CH
Catalog Number Description Qty
3448-020-01 3-D Culture 10X Cell Harvesting Buffer 1
3448-020-02 3-D Culture 10X Cell Wash Buffer 1