PARP In Vivo Pharmacodynamic Assay II


The HT PARP in vivo Pharmacodynamic Assay II Kit monitors in vivo PARP activity within cells based on levels of PAR.

Contract Services are also available.

Available Size(s): 96 tests Catalog Number: 4520-096-K Categories: , ,


Immobilized PAR monoclonal antibody in the wells of a 96-well plate captures cellular PAR and PAR attached to proteins. Incubation with a polyclonal PAR detecting antibody, followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of cellular PAR.


  • Pre-coated 96 well capture antibody plates
  • High signal to noise ratio
  • Detection sensitivity of 2 pg/ml of PAR
  • Broad linear dynamic range to 1,000 pg/ml
  • Reduced inter-assay variability
  • Validated assay that measures drug action on PARP in both in vivo and in vitro settings
  • 96 test size


  • Quantitation of PAR in peripheral blood mononuclear cells, tissue culture cells, and tumor lysates from different tissues, organs and xenografts.
  • Monitoring the efficacy of PARP inhibitors on PAR formation in vivo.
  • Verifying observations of enhanced cancer cell cytotoxicity arising from PARP inhibitor/anticancer drug combination therapy.
  • Facilitating development of PARP and PARG targeted therapeutics.


Material Safety Data Sheet(s)
msds_4520-096-01 PAR Standard 25 pg per ul
msds_4520-096-02 Sample Buffer
msds_4520-096-03 PAR Polyclonal Detecting Antibody
msds_4520-096-04 Goat anti-Rabbit IgG-HRP
msds_4520-096-05 Cell Lysis Reagent
msds_4520-096-06 DNase I 2 Units per
msds_4520-096-07 100X Magnesium Cation
msds_4520-096-08 Jurkat Cell Lysate Control Standard Low
msds_4520-096-09 Jurkat Cell Lysate Control Standard Medium
msds_4520-096-10 Jurkat Cell Lysate Control Standard High
msds_4520-096-11 Antibody Diluent
msds_4520-096-12 20 percent SDS

4520-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.
msds_4675-096-01 PARP PeroxyGlowA
msds_4675-096-02 PARP PeroxyGlowB

Catalog # 4520-096-K includes :
Catalog Number Description Qty
4520-096-01 PAR Standard, 25 pg/µl 1
4520-096-08 Jurkat Cell Lysate Control Standard, Low 1
4520-096-09 Jurkat Cell Lysate Control Standard, Medium 1
4520-096-10 Jurkat Cell Lysate Control Standard, High 1
4520-096-03 PAR Polyclonal Detecting Antibody 1
4520-096-04 Goat anti-Rabbit IgG-HRP 1
4520-096-06 DNase I, 2 Units/µl 1
4520-096-02 Sample Buffer 1
4520-096-05 Cell Lysis Reagent 1
4520-096-07 100X Magnesium Cation 1
4520-096-11 Antibody Diluent 1
4520-096-12 20% SDS 1
4520-096-P Pre-coated white 96-stripwell plate and 5 sealers 1
4675-096-01 PARP PeroxyGlow™ A 1
4675-096-02 PARP PeroxyGlow™ B 1

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“Efficacy of Carboplatin Alone and in Combination with
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“Olga Karginova, Marni B. Siegel, Amanda E.D. Van Swearingen, Allison M. Deal,
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Mol. Cell. Biol., Dec 2015; 35: 3934 – 3944.

“Phase I Study of Veliparib (ABT-888) Combined with Cisplatin and
Vinorelbine in Advanced Triple-Negative Breast Cancer and/or BRCA
Mutation–Associated Breast Cancer”

“Eve T. Rodler, Brenda F. Kurland, Melissa Griffin, Julie R. Gralow, Peggy Porter, Rosa F. Yeh, Vijayakrishna
K. Gadi, Jamie Guenthoer, Jan H. Beumer, Larissa Korde, Sandra Strychor, Brian F. Kiesel, Hannah M.
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Epstein-Barr Virus Oncoprotein LMP1 Mediates Epigenetic Changes in Host Gene Expression through PARP1
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