Superoxide Dismutase Assay Kit (Cuvette)

$528.00

The Superoxide Dismutase Assay analyzes Superoxide Dismutase (SOD) activity in cell extracts using cuvettes. Click here for our High-Throughput 96 well plate version of the assay.

Available Size(s): 100 reactions Catalog Number: 7500-100-K Category:

Description

In the Superoxide Dismutase Assay, ions generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert NBT to NBT-diformazan. NBT-diformazan absorbs light at 550 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of NBT-diformazan formation. The extent of reduction in the appearance of NBT-diformazan is a measure of SOD activity present in experimental samples. The assay is free of interference by other catalytic activities, and is ideal for assaying SOD in mammalian cell lysates. The kit contains the proper lysis buffer and the reagents needed for 100 experimental tests, 50 positive controls, and 50 negative controls. Unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals. Each assay requires only about five minutes, and after a simple calculation, the percent inhibition of the formation of NBT-diformazan by SOD is converted to the relative activity of the sample.

FEATURES:

  • Suitable for mammalian cells.
  • Each sample takes only 5 minutes.
  • Contains SOD for 50 positive controls.
  • Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)SOD.

APPLICATIONS :

Calculating SOD activity by measuring the reduction of NBT-diformazan in cell extracts.

 

Protocol(s)

protocol_7500-100-K

Material Safety Data Sheet(s)

msds_7500-100-01 Super Oxide Dismutase

msds_7500-100-02 25X SOD Reaction Buffer

msds_7500-100-03 Xanthine Solution

msds_7500-100-04 NBT Solution

msds_7500-100-05 XOD Solution

msds_7500-100-06 20X Cell Lysis Solution

Catalog # 7500-100-K includes :
Catalog Number Description Qty
7500-100-01 Super Oxide Dismutase 1
7500-100-05 XOD Solution 1
7500-100-02 25X SOD Reaction Buffer 1
7500-100-03 Xanthine Solution 1
7500-100-04 NBT Solution 1
7500-100-06 20X Cell Lysis Solution 1

Rosiglitazone Causes Cardiotoxicity via Peroxisome Proliferator-Activated Receptor {gamma}-Independent Mitochondrial Oxidative Stress in Mouse Hearts
Huamei He, Hai Tao, Hui Xiong, Sheng Zhong Duan, Francis X. McGowan, Jr., Richard M. Mortensen, and James A. Balschi
Toxicol. Sci., Apr 2014; 138: 468 – 481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468


Protective activity of a novel resveratrol analogue, HS-1793, against DNA damage in 137Cs-irradiated CHO-K1 cells

Jeong, M.H., Yang, K.M., Jeong, D.H., Lee, C.G., Oh, S.J., Jeong, S.K., Lee, K.W., Jo, Y.R. and Jo, W.S.
J. Radiat Res, May 2014; 55: 464 – 47
http://jrr.oxfordjournals.org/cgi/content/abstract/55/3/464