In the HT Superoxide Dismutase Assay, superoxide radical ions, generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert WST-1 to WST-1-formazan. WST-1-formazan absorbs light at 450 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of WST-1 formazan formation. The extent of reduction in the appearance of WST-1 formazan is a measure of SOD activity present in experimental samples. This assay is free of interference by other catalytic activities, and unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals.
- 96 well format for high throughput screening
- Assay performed in as little as 6 minutes
- Suitable for mammalian tissues and cell lysates
- Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD
Calculating relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan.