HT Superoxide Dismutase Assay Kit (96 Well Plate)

$561.00

The HT Superoxide Dismutase Assay analyzes Superoxide Dismutase (SOD) activity in cell extracts using a 96 well plate.  Click here for our cuvette version of the assay.

Available Size(s): 480 Wells Catalog Number: 7501-500-K Category:

Description

7501-500-k_calculation_worksheet

In the HT Superoxide Dismutase Assay, superoxide radical ions, generated from the conversion of xanthine to uric acid, and hydrogen peroxide by xanthine oxidase (XOD), convert WST-1 to WST-1-formazan. WST-1-formazan absorbs light at 450 nm. SODs reduce superoxide ion concentrations and thereby lower the rate of WST-1 formazan formation. The extent of reduction in the appearance of WST-1 formazan is a measure of SOD activity present in experimental samples. This assay is free of interference by other catalytic activities, and unlike some other assay kits for SOD, this system is not greatly disturbed by trace metals.

FEATURES:

  • 96 well format for high throughput screening
  • Assay performed in as little as 6 minutes
  • Suitable for mammalian tissues and cell lysates
  • Suitable for the assay of (Mn2+)-SOD, (Fe2+)-SOD, and (Cu/Zn)-SOD

APPLICATIONS:

Calculating relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan.

 

Protocol(s)

protocol_7501-500-K

Material Safety Data Sheet(s)

msds_7501-500-01 SOD Standard

msds_7501-500-02 10X SOD Buffer

msds_7501-500-03 Xanthine Oxidase XOD

msds_7501-500-04 10X Xanthine Solution

msds_7501-500-05 20 Triton X-100

msds_7501-500-06 WST-1 Reagent

7512-100-06 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 7501-500-K includes :
Catalog Number Description Qty
7501-500-01 SOD Standard 1
7501-500-03 Xanthine Oxidase (XOD) 1
7501-500-02 10X SOD Buffer 1
7501-500-04 10X Xanthine Solution 1
7501-500-05 20% Triton X-100 1
7501-500-06 WST-1 Reagent 1
7512-100-06 96-Well Plate 1