HT Glutathione Peroxidase Assay Kit

$495.00

Trevigen’s Glutathione Peroxidase Assay Kit measures glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.

Available Size(s): 5 x 96 Wells Catalog Number: 7512-100-K Category:

Description

7512-100-k_cuvette_assay_calculation_worksheet

7512-100-k_plate_assay_calculation_worksheet

Tetrameric Glutathione Peroxidase catalyzes the reduction of H2O2 to water, and organic peroxides to the corresponding stable alcohols, by using glutathione as a source of reducing equivalents. It requires selenium as a cofactor and contains a selenocysteine amino acid residue in the active site of each monomer that participates in the actual mechanism of the enzyme. Glutathione Reductase (GR), provided with each kit, then reduces the oxidized glutathione to complete the cycle. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the Glutathione Peroxidase activity in the sample.

FEATURES:

  • Suitable for a 96 well or cuvette format
  • Suitable for plasma, erythrocyte lysates, tissue homogenates, and cell lysates

APPLICATIONS:

Calculating glutathione peroxidase activity.

 

Protocol(s)

protocol_7512-100-K

Material Safety Data Sheet(s)

msds_7512-100-01 Glutathione Peroxidase

msds_7512-100-02 10X Assay Buffer

msds_7512-100-03 GSH + NADPH

msds_7512-100-04 Glutathione Reductase

msds_7512-100-05 Cumene Hydroperoxide

7512-100-06 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 7512-100-K includes :
Catalog Number Description Qty
7512-100-01 Glutathione Peroxidase 1
7512-100-02 10X Assay Buffer 1
7512-100-03 GSH+NADPH 1
7512-100-04 Glutathione Reductase 1
7512-100-05 Cumene Hydroperoxide 1
7512-100-06 96-well plates 1

Glutathione-Mediated Detoxification of Halobenzoquinone Drinking Water Disinfection Byproducts in T24 Cells
Jinhua Li, Wei Wang, Hongquan Zhang, X. Chris Le, and Xing-Fang Li
Toxicol. Sci., Oct 2014; 141: 335 – 343.
http://toxsci.oxfordjournals.org/cgi/content/abstract/141/2/335