Trevigen’s Glutathione Assay utilizes a carefully optimized enzymatic recycling method, which employs Glutathione Reductase, for the quantification of glutathione. The sulfhydryl group of glutathione reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow-colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by Glutathione Reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to the concentration of GSH in the sample. Thus, measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of the amount of glutathione in the sample.
- Suitable for mammalian cells, tissue, blood, plasma and other bodily fluids.
- Formatted for 96-well plates.
- Contains sufficient reagents to assay 384 data points or to determine GSH in:
- 123 experimental samples, each performed in triplicate, plus one GSH standard curve.
- 108 experimental samples, each performed in triplicate, plus 4 GSH standard curves.
- 88 experimental samples, each performed in triplicate, plus 8 GSH standard curves.
- Includes procedure for determining oxidized and reduced glutathione.
Calculating total, reduced, and oxidized glutathione concentrations.
Material Safety Data Sheet(s)
msds_7511-100-01 Glutathione Reductase
msds_7511-100-02 25X Assay Buffer
msds_7511-100-04 Reaction Mix
7511-100-05 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.
msds_7511-100-06 4 uM GSSG
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