HT Glutathione Assay Kit (Total Glutathione)

$346.00

Trevigen’s High-Throughput Glutathione Assay Kit quantifies the amount of glutathione in mammalian cells, tissue, blood, plasma and other bodily fluids using a 96 well plate.

Available Size(s): 384 Tests Catalog Number: 7511-100-K Category:

Description

7511-100-k_calculation_worksheet

Trevigen’s Glutathione Assay utilizes a carefully optimized enzymatic recycling method, which employs Glutathione Reductase, for the quantification of glutathione. The sulfhydryl group of glutathione reacts with DTNB (5,5’-dithiobis-2-nitrobenzoic acid, Ellman’s reagent) and produces a yellow-colored 5-thio-2-nitrobenzoic acid (TNB). The mixed disulfide, GSTNB (between GSH and TNB) that is concomitantly produced, is reduced by Glutathione Reductase to recycle the GSH and produce more TNB. The rate of TNB production is directly proportional to the concentration of GSH in the sample. Thus, measurement of the absorbance of TNB at 405 or 412 nm provides an accurate estimation of the amount of glutathione in the sample.

FEATURES:

  • Suitable for mammalian cells, tissue, blood, plasma and other bodily fluids.
  • Formatted for 96-well plates.
  • Contains sufficient reagents to assay 384 data points or to determine GSH in:
  1. 123 experimental samples, each performed in triplicate, plus one GSH standard curve.
  2. 108 experimental samples, each performed in triplicate, plus 4 GSH standard curves.
  3. 88 experimental samples, each performed in triplicate, plus 8 GSH standard curves.
  • Includes procedure for determining oxidized and reduced glutathione.

APPLICATIONS:

Calculating total, reduced, and oxidized glutathione concentrations.

Protocol(s)

protocol_7511-100-K

Material Safety Data Sheet(s)

msds_7511-100-01 Glutathione Reductase

msds_7511-100-02 25X Assay Buffer

msds_7511-100-04 Reaction Mix

7511-100-05 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_7511-100-06 4 uM GSSG

Catalog # 7511-100-K includes :
Catalog Number Description Qty
7511-100-01 Glutathione Reductase 1
7511-100-02 25X Assay Buffer 1
7511-100-04 Reaction Mix 1
7511-100-05 96-Well Plates 1
7511-100-06 4 µM GSSG 1

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Huamei He, Hai Tao, Hui Xiong, Sheng Zhong Duan, Francis X. McGowan, Jr., Richard M. Mortensen, and James A. Balschi
Toxicol. Sci., Apr 2014; 138: 468 – 481.
http://toxsci.oxfordjournals.org/cgi/content/abstract/138/2/468

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“Yusuke Shono, Andrea Z. Tuckett, Hsiou-Chi Liou, Ekaterina Doubrovina, Enrico Derenzini, Samedy Ouk,Jennifer J. Tsai, Odette M. Smith, Emily R. Levy, Fabiana M. Kreines, Carly G.K. Ziegler, Mary I. Scallion, Mikhail Doubrovin, Glenn Heller, Anas Younes, Richard J. O’Reilly, Marcel R.M. van den Brink, and Johannes L. Zakrzewski”
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Increased VEGF-A promotes multiple distinct aging diseases of the eye through shared pathomechanisms
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