HT 8-oxo-dG ELISA Kit II

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The HT 8-oxo-dG ELISA Kit II quantifies 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.

Clear
Available Size(s): 2 x 96 Tests, 96 tests Catalog Number: 4380-096-K Category:

Description

4380-096-k_calculation_worksheet

An 8-OHdG monoclonal antibody binds competitively to 8-oxo-dG immobilized on pre-coated wells and in a sample solution. Detection of the retained antibody is performed using an HRP conjugate and colorimetric substrate. Product formation is inversely proportional to the amount of 8-oxo-dG present in the sample.  The anti-8-oxo-dG monoclonal antibody may be purchased separately.

FEATURES:

  • Colorimetric, non-radioactive format
  • High throughput 96 strip wells
  • Dynamic range from 3.13 nM to 200 nM (0.89 ng/ml to 56.7 ng/ml)
  • Sensitivity at 2 nM (0.57 ng/ml) 8-OHdG

APPLICATIONS:
For the detection and quantitation of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in DNA, plasma, urine and saliva samples.

Protocol(s)

protocol_4380-096-K

protocol_4380-192-K

Material Safety Data Sheet(s)

msds_4380-096-01 8-OHdG Standard 20 uM

msds_4380-096-02 Assay Diluent

msds_4380-096-03 8-OHdG Monoclonal Antibody

msds_4380-096-04 Goat anti-Mouse IgG-HRP

msds_4380-096-05 100X Cations

msds_4380-096-06 DNase I 5 units per

msds_4380-096-07 Alkaline Phosphatase

msds_4822-96-08 TACS-Sapphire

4380-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 4380-096-K includes :
Catalog Number Description Qty
4380-096-01 8-OHdG Standard, 20 µM 1
4380-096-03 8-OHdG Monoclonal Antibody 1
4380-096-04 Goat anti-Mouse IgG-HRP 1
4380-096-06 DNase I (5 Units/µl) 1
4380-096-07 Alkaline Phosphatase (1 Unit/µl) 1
4380-096-02 Assay Dilutent 1
4380-096-05 100X Cations 1
4380-096-P Pre-coated 96-stripwell plate 1
4822-96-08 TACS-Sapphire™ 1

 

Catalog # 4380-192-K includes :
Catalog Number Description Qty
4380-096-01 8-OHdG Standard, 20 µM 2
4380-096-03 8-OHdG Monoclonal Antibody 2
4380-096-04 Goat anti-Mouse IgG-HRP 2
4380-096-06 DNase I (5 Units/µl) 2
4380-096-07 Alkaline Phosphatase (1 Unit/µl) 2
4380-096-02 Assay Dilutent 2
4380-096-05 100X Cations 2
4380-096-P Pre-coated 96-stripwell plate 2
4822-96-08 TACS-Sapphire™ 2

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