Thermostable TDG Protein and 10X REC™ Buffer 4

TDG is a thermostable thymine DNA glycosylase from Methanobacterium thermoautotrophicum. The optimal temperature for the enzyme is 65°C. The enzyme lacks significant AP lyase or endonuclease activity. TDG works effectively in heteroduplex analysis to detect C to T transitions.

Available Size(s): 500 units, 1ml Catalog Number: 4070-500-EB Category:

Description

CONCENTRATION
500 units per vial
SOURCE
Purified from E.  coli containing a recombinant plasmid harboring the Methanobacterium thermoautotrophicum TDG gene.
SUBSTRATE SPECIFICITY
Recognizes T/G mismatches in duplex DNA and cleaves the strand with the T. Also, recognizes G/G mismatches if at least one nearest neighbor is an A or T and nicks one strand or the other.
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
DNA Repair Assays

Protocol(s)

protocol_4070-500-EB

Material Safety Data Sheet(s)

msds_4070-500-01 Thermostable TDG Enzyme

msds_3900-500-04 10X REC Reaction Buffer 4

Catalog # 4070-500-EB includes :
Catalog Number Description Qty
3900-500-04 10X REC™ Reaction Buffer 4 1
4070-500-01 Thermostable TDG Enzyme 1

DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B
Yang Fu, Fumiaki Ito, Gewen Zhang, Braulio Fernandez, Hanjing Yang, and Xiaojiang S. Chen
Biochem. J., Oct 2015; 471: 25 – 35.
http://www.biochemj.org/cgi/content/abstract/471/1/25

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