100 units per vial
Purified from E. coli containing a recombinant plasmid harboring the α-hOGG1 gene (nuclear protein).
The catalytic activity of hOGG1 is dependent upon the base the 8-oxoG is paired with in the order of C>T>G, A. hOGG1 is also catalytically active when FaPy is paired with C. FaPy is only repaired when base paired to cytosine.
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
DNA Repair Assays
“Influence of Oxidized Purine Processing on Strand
Directionality of Mismatch Repair”
Simone Repmann, Maite Olivera-Harris, and Josef Jiricny
J. Biol. Chem., Apr 2015; 290: 9986 – 9999
Formation and Repair of Mismatches Containing Ribonucleotides and Oxidized Bases at Repeated DNA Sequences
Piera Cilli, Anna Minoprio, Cecilia Bossa, Margherita Bignami, and Filomena Mazzei
J. Biol. Chem., Oct 2015; 290: 26259 – 26269.
Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine to induce CAG/CTG repeat instability
Piera Cilli, Ilenia Ventura, Anna Minoprio, Ettore Meccia, Alberto Martire, Samuel H. Wilson, Margherita Bignami, and Filomena Mazzei
Nucleic Acids Res., Mar 2016; 10.1093/nar/gkw170
“Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine
to induce CAG/CTG repeat instability”
“Piera Cilli, Ilenia Ventura, Anna Minoprio, Ettore Meccia, Alberto Martire, Samuel H. Wilson, Margherita Bignami, and Filomena Mazzei”
Nucleic Acids Res., Jun 2016; 44: 5190 – 5203.
“Weak silica nanomaterial-induced genotoxicity can be explained by
indirect DNA damage as shown by the OGG1-modified comet assay and
Stefan Pfuhler, Thomas R. Downs, Ashley J. Allemang, Yuching Shan, and Meredith E. Crosby Mutagenesis, Nov 2016; 10.1093/mutage/gew064.