Human 8-oxoGuanine DNA Glycosylase and 10X REC™ Buffer 6

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Human 8-oxo-guanine DNA glycosylase (hOGG1) catalyzes the removal of the DNA adducts 7,8-dihydro-8-oxoguanine (8-oxoG) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FaPy) through cleavage of the DNA phosphodiester bond following Schiff base chemistry. hOGG1 does not recognize the C=O of 8-oxoG as expected, but rather recognizes a proton on N7 of the nucleotide. By mispairing with adenine during replication, 8-oxoG gives rise to G:C to T:A transversions, a frequent somatic mutation in human cancers. In contrast, a FaPy lesion leads to termination of replication and, therefore, is not considered a pre-mutagenic lesion.

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Available Size(s): 100 units, 5 x 100 units, 1 ml Catalog Number: 4130-100-EB Category:

Description

CONCENTRATION
100 units per vial
SOURCE
Purified from E. coli containing a recombinant plasmid harboring the α-hOGG1 gene (nuclear protein).
SUBSTRATE SPECIFICITY
The catalytic activity of hOGG1 is dependent upon the base the 8-oxoG is paired with in the order of C>T>G, A.  hOGG1 is also catalytically active when FaPy is paired with C. FaPy is only repaired when base paired to cytosine.
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
DNA Repair Assays
FLARE™

Protocol(s)

protocol_4130-100-EB

protocol_4130-500-EB

Material Safety Data Sheet(s)

msds_4130-100-01 hOGG1 Enzyme

msds_3900-500-06 10X REC Reaction Buffer 6

Catalog # 4130-100-EB includes : 
Catalog Number Description Qty
3900-500-06 10X REC™ Reaction Buffer 6 1
4130-100-01 E. coli hOGG1 1

 

Catalog # 4130-500-EB includes : 
Catalog Number Description Qty
3900-500-06 10X REC™ Reaction Buffer 6 1
4130-100-01 E. coli hOGG1 5

 

“Influence of Oxidized Purine Processing on Strand
Directionality of Mismatch Repair”

Simone Repmann, Maite Olivera-Harris, and Josef Jiricny
J. Biol. Chem., Apr 2015; 290: 9986 – 9999
http://www.jbc.org/cgi/content/abstract/290/16/9986

Formation and Repair of Mismatches Containing Ribonucleotides and Oxidized Bases at Repeated DNA Sequences

Piera Cilli, Anna Minoprio, Cecilia Bossa, Margherita Bignami, and Filomena Mazzei
J. Biol. Chem., Oct 2015; 290: 26259 – 26269.
http://www.jbc.org/cgi/content/abstract/290/43/26259

Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine to induce CAG/CTG repeat instability
Piera Cilli, Ilenia Ventura, Anna Minoprio, Ettore Meccia, Alberto Martire, Samuel H. Wilson, Margherita Bignami, and Filomena Mazzei
Nucleic Acids Res., Mar 2016; 10.1093/nar/gkw170
http://nar.oxfordjournals.org/cgi/content/abstract/gkw170v1

“Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine
to induce CAG/CTG repeat instability”

“Piera Cilli, Ilenia Ventura, Anna Minoprio, Ettore Meccia, Alberto Martire, Samuel H. Wilson, Margherita Bignami, and Filomena Mazzei”
Nucleic Acids Res., Jun 2016; 44: 5190 – 5203.
http://nar.oxfordjournals.org/cgi/content/abstract/44/11/5190

“Weak silica nanomaterial-induced genotoxicity can be explained by
indirect DNA damage as shown by the OGG1-modified comet assay and
genomic analysis”

Stefan Pfuhler, Thomas R. Downs, Ashley J. Allemang, Yuching Shan, and Meredith E. Crosby Mutagenesis, Nov 2016; 10.1093/mutage/gew064.
http://mutage.oxfordjournals.org/cgi/content/abstract/gew064v1