E. coli Mut Y DNA Glycosylase and 10X REC™ Buffer 4

$319.00

E. coli MutY DNA Glycosylase (MutY) acts together with E.coli Fpg to prevent the potentially mutagenic consequences of 8-oxo-dG lesions. 8-oxo-dG lesions escaping repair by Fpg frequently pair with A during DNA replication, producing an 8-oxo-dG:A mispair. MutY removes the A from this base pair to initiate base excision repair. In the absence of MutY, DNA replication after an 8-oxo-dG:A mismatch results in thymine incorporation opposite the adenine in one of the daughter strands, creating a fixed mutation. MutY has an associated AP lyase activity.

Available Size(s): 5000 units, 1 ml Catalog Number: 4000-500-EB Category:

Description

CONCENTRATION
5000 units per vial
SOURCE
Purified from E. coli containing a recombinant plasmid harboring the E.coli MutY gene.
SUBSTRATE SPECIFICITY
8-oxo-dG:A mispair
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
DNA Repair Assays
FLARE™

Protocol(s)

protocol_4000-500-EB

Material Safety Data Sheet(s)

msds_4000-500-01 E. coli Mut Y

msds_3900-500-04 10X REC Reaction Buffer 4

Catalog # 4000-500-EB includes :
Catalog Number Description Qty
3900-500-04 10X REC™ Buffer 4 1
4000-500-01 E. coli Mut Y DNA Glycosylase 1