E. coli Formamidopyrimidine-DNA Glycosylase and 10X REC™ Buffer 10

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E. coli Formamidopyrimidine-DNA Glycosylase (Fpg) releases damaged bases preferentially from duplex DNA. It has an associated class I AP lyase activity, leaving both 3′ and 5′ phosphoryl groups. This results from a β-elimination reaction at the AP sites, producing a single nucleotide gap in the DNA. The enzyme has a molecular weight of 30.2 kDa.

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Available Size(s): 2 x 500 units, 1 ml, 500 units, 1 ml Catalog Number: 4040-100-EB Category:

Description

CONCENTRATION
500 units per vial
SOURCE
Purified from E. coli containing a recombinant plasmid harboring the E. coli fpg gene.
SUBSTRATE SPECIFICITY
Open ring forms of 7-methylguanine, 8-oxoguanine, 5-hydroxycytosine, 5-hydroxyuracil, aflatoxin-bound imidazole-ring-opened guanine, and imidazole ring opened N-2-aminofluorene-C8-guanine.
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
DNA Repair Assays
FLARE™

Protocol(s)

protocol_4040-100-EB

protocol_4040-500-EB

Material Safety Data Sheet(s)

msds_4040-100-01 E. coli Fpg

msds_3900-500-10 10X REC Reaction Buffer 10

Catalog # 4040-500-EB includes : 
Catalog Number Description Qty
3900-500-10 10X REC™ Buffer 10 1
4040-100-01 E. coli Fpg 5
Catalog # 4040-100-EB includes :
Catalog Number Description Qty
3900-500-10 10X REC™ Buffer 10 1
4040-100-01 E. coli Fpg 1

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