DPO-4 polymerase with 10X REC™ Buffer 15 and Cations

Sulfolobus solfataricus DNA polymerase IV (Dpo4) is a thermophilic Y-family DNA polymerase capable of performing translesion synthesis. Dpo4 has a molecular weight of 42 kDa and is 95% pure by SDS PAGE.

Available Size(s): 10μg, 1ml, 50μg, 1ml Catalog Number: 4150-010-EB Category:

Description

CONCENTRATION
10 or 50 µg per vial
SOURCE
Purified from E. coli containing a recombinant plasmid harboring the S. solfataricus Dpo4 gene.
SUBSTRATE SPECIFICITY
Translesion synthesis across AP sites, cis-syn thymine-thymine dimer, 6-4 photoproducts, cisplatinum- and acetyl aminofluorene guanine adducts.
STORAGE
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
APPLICATIONS
Allows for coupling to PCR for amplification of damaged DNA.

Protocol(s)

protocol_4150-010-EB

protocol_4150-050-EB

Material Safety Data Sheet(s)

msds_4150-010-01 DPO4

msds_4150-010-02 100mM MgCl2

msds_4150-010-03 50 mM MnCl2

msds_3900-500-15 10X Reaction Buffer 15

msds_4150-050-01 DPO4

Catalog # 4150-050-EB includes : 
Catalog Number Description Qty
3900-500-15 10X REC™ Buffer 15 1
4150-010-02 100 mM MgCl2 1
4150-010-03 50 mM MnCl2 1
4150-050-01 S. solfataricus Dpo4 1
Catalog # 4150-010-EB includes :
Catalog Number Description Qty
3900-500-15 10X REC™ Buffer 15 1
4150-010-02 100 mM MgCl2 1
4150-010-03 50 mM MnCl2 1
4150-010-01 S. solfataricus Dpo4 1

Structural and biochemical impact of C8-aryl-guanine adducts within the NarI recognition DNA sequence: influence of aryl ring size on targeted and semi-targeted mutagenicity
Michael Sproviero, Anne M.R. Verwey, Katherine M. Rankin, Aaron A. Witham, Dmitriy V. Soldatov, Richard A. Manderville, Mostafa I. Fekry, Shana J. Sturla, Purshotam Sharma, and Stacey D. Wetmore Nucleic Acids Res., Dec 2014; 42: 13405 – 13421.
Nucleic Acids Res., Dec 2014; 42: 13405 – 13421
http://nar.oxfordjournals.org/cgi/content/abstract/42/21/13405