10 or 50 µg per vial
Purified from E. coli containing a recombinant plasmid harboring the S. solfataricus Dpo4 gene.
Translesion synthesis across AP sites, cis-syn thymine-thymine dimer, 6-4 photoproducts, cisplatinum- and acetyl aminofluorene guanine adducts.
Store at -20°C in a manual defrost freezer. For long-term storage, freeze at -80°C in working aliquots.
Allows for coupling to PCR for amplification of damaged DNA.
Structural and biochemical impact of C8-aryl-guanine adducts within the NarI recognition DNA sequence: influence of aryl ring size on targeted and semi-targeted mutagenicity
Michael Sproviero, Anne M.R. Verwey, Katherine M. Rankin, Aaron A. Witham, Dmitriy V. Soldatov, Richard A. Manderville, Mostafa I. Fekry, Shana J. Sturla, Purshotam Sharma, and Stacey D. Wetmore Nucleic Acids Res., Dec 2014; 42: 13405 – 13421.
Nucleic Acids Res., Dec 2014; 42: 13405 – 13421