CometAssay® Kit (96 well, 1 slide)

CometAssay® 96 Kit includes one specially treated CometSlide™ (96 Well) and sufficient reagents (Lysis Solution, LMAgarose, and 200 mM EDTA pH 8) for 96 test samples.

Available Size(s): 96 Samples Catalog Number: 4253-096-K Category:

Description

FEATURES:

  • Specially treated slides to promote agarose adherence
  • Ready to use low melt agarose (LMAgarose) in convenient size
  • Lysis Solution suitable for either alkaline or neutral comet assay
  • Optimized electrophoresis conditions

APPLICATIONS:

  • Applicable for the analysis of either single- or double-strand DNA breaks
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens

Protocol(s)

protocol_4253-096-K

Material Safety Data Sheet(s)

msds_4250-050-02 LMAgarose

msds_4250-050-04 200mM EDTA

msds_4250-010-01 Lysis Solution

4253-096-03 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 4253-096-K includes :
Catalog Number Description Qty
4250-010-01 CometAssay® Lysis Solution 1
4250-050-02 CometAssay® LMAgarose 1
4250-050-04 200 mM EDTA, pH 10 1
4253-096-03 CometSlide™ (96 Well) 1

 

“HT-COMET: a novel automated approach for high throughput
assessment of human sperm chromatin quality”

Océane Albert, Wolfgang E. Reintsch, Peter Chan, and Bernard Robaire
Hum. Reprod., May 2016; 31: 938 – 946.
http://humrep.oxfordjournals.org/cgi/content/abstract/31/5/938

HUWE1 interacts with PCNA to alleviate replication stress

Katherine N Choe, Claudia M Nicolae, Daniel Constantin, Yuka Imamura Kawasawa, Maria Rocio Delgado-Diaz, Subhajyoti De, Raimundo Freire, Veronique AJ Smits, and George-Lucian Moldovan
EMBO Rep., May 2016; 10.15252/embr.201541685.
http://EMBOr.embopress.org/cgi/content/abstract/embr.201541685v1

PKCα and HMGB1 antagonistically control hydrogen peroxide-induced poly-ADP-ribose formation
Anneli Andersson, Andrej Bluwstein, Nitin Kumar, Federico Teloni, Jens Traenkle, Michael Baudis, Matthias Altmeyer, and Michael O. Hottiger
Nucleic Acids Res., May 2016; 10.1093/nar/gkw442.
http://nar.oxfordjournals.org/cgi/content/abstract/gkw442v1

Disruption of DNA Damage-Response by Propyl Gallate and 9-Aminoacridine
Shun Matsuda, Yoko Matsuda, Shin-Ya Yanagisawa, Masae Ikura, Tsuyoshi Ikura, and Tomonari Matsuda
Toxicol. Sci., Jun 2016; 151: 224 – 235.
http://toxsci.oxfordjournals.org/cgi/content/abstract/151/2/224

“A ubiquitylation site in Cockayne syndrome B required for repair of
oxidative DNA damage, but not for transcription-coupled nucleotide
excision repair”

“Michael Ranes, Stefan Boeing, Yuming Wang, Franziska Wienholz, Hervé Menoni, Jane Walker, Vesela
Encheva, Probir Chakravarty, Pierre-Olivier Mari, Aengus Stewart, Giuseppina Giglia-Mari, Ambrosius P.
Snijders, Wim Vermeulen, and Jesper Q. Svejstrup”
Nucleic Acids Res., Jun 2016; 44: 5246 – 5255.
http://nar.oxfordjournals.org/cgi/content/abstract/44/11/5246

“Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells”
“Meetu Kaushik Tiwari, Nneoma Adaku, Natoya Peart, and Faye A. Rogers”
Nucleic Acids Res., Jun 2016; 10.1093/nar/gkw515
http://nar.oxfordjournals.org/cgi/content/abstract/gkw515v1

“Differences in quantification of DNA double-strand breaks assessed by 53BP1/ H2AX focus formation assays and the comet assay in mammalian cells treated with irradiation and N-acetyl-L-cysteine”
Tomomi Kurashige, Mika Shimamura, and Yuji Nagayama
J Radiat Res, Jun 2016; 57: 312 – 317
http://jrr.oxfordjournals.org/cgi/content/abstract/57/3/312

“HUWE1 interacts with PCNA to alleviate replication stress”
“Katherine N Choe, Claudia M Nicolae, Daniel Constantin, Yuka Imamura Kawasawa, Maria Rocio
Delgado-Diaz, Subhajyoti De, Raimundo Freire, Veronique AJ Smits, and George-Lucian Moldovan”
EMBO Rep., Jun 2016; 17: 874 – 886.
http://EMBOr.embopress.org/cgi/content/abstract/17/6/874

“DNA Damage Response and DNA Repair in Skeletal Myocytes From a
Mouse Model of Spinal Muscular Atrophy”

Saniya Fayzullina and Lee J. Martin
J Neuropathol Exp Neurol, Jul 2016; 10.1093/jnen/nlw064
http://jnen.oxfordjournals.org/cgi/content/abstract/nlw064v1

“Overexpression of HPV16 E6* Alters β-Integrin and Mitochondrial
Dysfunction Pathways in Cervical Cancer Cells”

“WHITNEY EVANS, MARIA FILIPPOVA, VALERY FILIPPOV, SVETLANA BASHKIROVA, GUANGYU ZHANG,MARK E. REEVES, and PENELOPE DUERKSEN-HUGHES”
Cancer Genomics Proteomics, Jul 2016; 13: 259 – 273
http://cgp.iiarjournals.org/cgi/content/abstract/13/4/259

“Androgen Deprivation Followed by Acute Androgen Stimulation
Selectively Sensitizes AR-Positive Prostate Cancer Cells to Ionizing
Radiation”

“Mohammad Hedayati, Michael C. Haffner, Jonathan B. Coulter, Raju R. Raval, Yonggang Zhang, Haoming
Zhou, Omar Mian, Emma J. Knight, Nina Razavi, Susan Dalrymple, John T. Isaacs, Aileen Santos, Russell
Hales, William G. Nelson, Srinivasan Yegnasubramanian, and Theodore L. DeWeese”
Clin. Cancer Res., Jul 2016; 22: 3310 – 3319
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/13/3310

“NQO1-Mediated Tumor-Selective Lethality and Radiosensitization for
Head and Neck Cancer”

“Long-Shan Li, Srilakshmi Reddy, Zhen-Hua Lin, Shuangping Liu, Hyunsil Park, Stephen G. Chun, William G.
Bornmann, Joel Thibodeaux, Jingsheng Yan, Gaurab Chakrabarti, Xian-Jin Xie, Baran D. Sumer, David A.
Boothman, and John S. Yordy”
Mol. Cancer Ther., Jul 2016; 15: 1757 – 1767
http://mct.aacrjournals.org/cgi/content/abstract/15/7/1757

“HOXC10 Expression Supports the Development of Chemotherapy
Resistance by Fine Tuning DNA Repair in Breast Cancer Cells”

“Helen Sadik, Preethi Korangath, Nguyen K. Nguyen, Balazs Gyorffy, Rakesh Kumar, Mohammad
Hedayati, Wei Wen Teo, Sunju Park, Hardik Panday, Teresa Gonzalez Munoz, Otilia Menyhart, Nilay Shah, Raj K. Pandita, Jenny C. Chang, Theodore DeWeese, Howard Y. Chang, Tej K. Pandita, and Saraswati Sukumar”
Cancer Res., Aug 2016; 76: 4443 – 4456.
http://cancerres.aacrjournals.org/cgi/content/abstract/76/15/4443

“A panel of in vitro tests to evaluate genotoxic and morphological
neoplastic transformation potential on Balb/3T3 cells by pristine and remediated titania and zirconia nanoparticles”

“Andrea Stoccoro, Sebastiano Di Bucchianico, Chiara Uboldi, Fabio Coppedè, Jessica Ponti, Claudia Placidi,
Magda Blosi, Simona Ortelli, Anna Luisa Costa, and Lucia Migliore”
Mutagenesis, Sep 2016; 31: 511 – 529.
http://mutage.oxfordjournals.org/cgi/content/abstract/31/5/511

Distribution, quantification and toxicity of cinnamaldehyde in electronic cigarette refill fluids and aerosols

Rachel Z Behar, Wentai Luo, Sabrina C Lin, Yuhuan Wang, Jackelyn Valle, James F Pankow, and Prue Talbot
Tob. Control, Sep 2016; 10.1136/tobaccocontrol-2016-053224
http://tobaccocontrol.bmj.com/cgi/content/abstract/tobaccocontrol-2016-053224v1

Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells
Meetu Kaushik Tiwari, Nneoma Adaku, Natoya Peart, and Faye A. Rogers
Nucleic Acids Res., Sep 2016; 44: 7742 – 7754
http://nar.oxfordjournals.org/cgi/content/abstract/44/16/7742

“The MCT4 Gene: A Novel, Potential Target for Therapy of Advanced Prostate Cancer”
“Stephen Yiu Chuen Choi, Hui Xue, Rebecca Wu, Ladan Fazli, Dong Lin, Colin C. Collins, Martin E. Gleave,Peter W. Gout, and Yuzhuo Wang”
Clin. Cancer Res., Jun 2016; 22: 2721 – 2733
http://clincancerres.aacrjournals.org/cgi/content/abstract/22/11/2721