CometAssay® Kit (25 x 2 well slides)

$247.00

CometAssay® Kit includes twenty-five specially treated CometSlides™ (2 Well) and sufficient reagents (Lysis Solution, LMAgarose, and 200 mM EDTA pH 8) for 50 test samples.

Available Size(s): 50 samples Catalog Number: 4250-050-K Category:

Description

FEATURES:

  • Specially treated slides to promote agarose adherence
  • Ready to use low melt agarose (LMAgarose) in convenient size
  • Lysis Solution suitable for either alkaline or neutral comet assay
  • Optimized electrophoresis conditions

APPLICATIONS:

  • Applicable for the analysis of either single- or double-strand DNA breaks
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens

Protocol(s)

protocol_4250-050-K

Material Safety Data Sheet(s)

msds_4250-050-01 Lysis Solution

msds_4250-050-02 LMAgarose

4250-050-03 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4250-050-04 200mM EDTA

USP1 targeting impedes GBM growth by inhibiting stem cell
Jin-Ku Lee, Nakho Chang, Yeup Yoon, Heekyoung Yang, Heejin Cho, Eunhee Kim, Yongjae Shin, Wonyoung Kang, Young Taek Oh, Gyeong In Mun, Kyeung Min Joo, Do-Hyun Nam, and Jeongwu Lee
Neuro Oncology, Jan 2016; 18: 37 – 47.
http://neuro-oncology.oxfordjournals.org/cgi/content/abstract/18/1/37

DNA double-strand break repair: a theoretical framework and its application
Philip J. Murray, Bart Cornelissen, Katherine A. Vallis, and S. Jon Chapman
J R Soc Interface, Jan 2016; 13: 20150679.
http://rsif.royalsocietypublishing.org/cgi/content/abstract/13/114/20150679

The Opposing Actions of Arabidopsis CHROMOSOME TRANSMISSION FIDELITY7 and WINGS APART-LIKE1 and 2 Differ in Mitotic and Meiotic Cells
Kuntal De, Pablo Bolaños-Villegas, Sayantan Mitra, Xiaohui Yang, Garret Homan, Guang-Yuh Jauh, and Christopher A. Makaroff
PLANT CELL, Feb 2016; 28: 521 – 536
http://www.plantcell.org/cgi/content/abstract/28/2/521

“Intracellular accumulation of indium ions released from nanoparticles induces oxidative stress, proinflammatory response and DNA damage”
Yosuke Tabei, Akinari Sonoda, Yoshihiro Nakajima, Vasudevanpillai Biju, Yoji Makita, Yasukazu Yoshida, and Masanori Horie
J. Biochem., Feb 2016; 159: 225 – 237
http://jb.oxfordjournals.org/cgi/content/abstract/159/2/225

“Long-term exposure to diesel engine exhaust induces primary DNA damage: a population-based study”
Huawei Duan, Xiaowei Jia, Qingfeng Zhai, Lu Ma, Shan Wang, Chuanfeng Huang, Haisheng Wang, Yong Niu, Xue Li, Yufei Dai, Shanfa Yu, Weimin Gao, Wen Chen, and Yuxin Zheng
Occup. Environ. Med., Feb 2016; 73: 83 – 90.
http://oem.bmj.com/cgi/content/abstract/73/2/83

Linking Cancer Metabolism to DNA Repair and Accelerated Senescence
Elena V. Efimova, Satoe Takahashi, Noumaan A. Shamsi, Ding Wu, Edwardine Labay, Olesya A. Ulanovskaya, Ralph R. Weichselbaum, Sergey A. Kozmin, and Stephen J. Kron
Mol. Cancer Res., Feb 2016; 14: 173 – 184.
http://mcr.aacrjournals.org/cgi/content/abstract/14/2/173

DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcriptionblocking topoisomerase I lesions
Agnese Cristini, Joon-Hyung Park, Giovanni Capranico, Gaëlle Legube, Gilles Favre, and Olivier Sordet
Nucleic Acids Res., Feb 2016; 44: 1161 – 1178
http://nar.oxfordjournals.org/cgi/content/abstract/44/3/1161

Requirement for human Mps1/TTK in oxidative DNA damage repair and cell survival through MDM2 phosphorylation
Zheng-Cheng Yu, Yi-Fu Huang, and Sheau-Yann Shieh
Nucleic Acids Res., Feb 2016; 44: 1133 – 1150
http://nar.oxfordjournals.org/cgi/content/abstract/44/3/1133

Cytokine overproduction and crosslinker hypersensitivity are unlinked in Fanconi anemia macrophages
Michael R. Garbati, Laura E. Hays, R. Keaney Rathbun, Nathaniel Jillette, Kathy Chin, Muhsen Al-Dhalimy, Anupriya Agarwal, Amy E. Hanlon Newell, Susan B. Olson, and Grover C. Bagby, Jr.
J. Leukoc. Biol., Mar 2016; 99: 455 – 465
http://www.jleukbio.org/cgi/content/abstract/99/3/455

The Role of HMGB1 in Radioresistance of Bladder Cancer
Sanhita Shrivastava, Jose Joao Mansure, Wael Almajed, Fabio Cury, Gerardo Ferbeyre, Marija Popovic, Jan Seuntjens, and Wassim Kassouf
Mol. Cancer Ther., Mar 2016; 15: 471 – 479
http://mct.aacrjournals.org/cgi/content/abstract/15/3/471

Differences in quantification of DNA double-strand breaks assessed by 53BP1/ H2AX focus formation assays and the comet assay in mammalian cells treated with irradiation and N-acetyl-L-cysteine
Tomomi Kurashige, Mika Shimamura, and Yuji Nagayama
J Radiat Res, Mar 2016; 10.1093/jrr/rrw001.
http://jrr.oxfordjournals.org/cgi/content/abstract/rrw001v1

Sequential Combination Therapy of CDK Inhibition and Doxorubicin Is Synthetically Lethal in p53-Mutant Triple-Negative Breast Cancer
Natalie A. Jabbour-Leung, Xian Chen, Tuyen Bui, Yufeng Jiang, Dong Yang, Smruthi Vijayaraghavan, Mark J. McArthur, Kelly K. Hunt, and Khandan Keyomarsi
Mol. Cancer Ther., Apr 2016; 15: 593 – 607.
http://mct.aacrjournals.org/cgi/content/abstract/15/4/593

“miR-155 Overexpression Promotes Genomic Instability by Reducing
High-fidelity Polymerase Delta Expression and Activating Error-Prone DSB Repair”

Jennifer R. Czochor, Parker Sulkowski, and Peter M. Glazer
Mol. Cancer Res., Apr 2016; 14: 363 – 373.
http://mcr.aacrjournals.org/cgi/content/abstract/14/4/363

“A panel of in vitro tests to evaluate genotoxic and morphological
neoplastic transformation potential on Balb/3T3 cells by pristine and remediated titania and zirconia nanoparticles”

Andrea Stoccoro, Sebastiano Di Bucchianico, Chiara Uboldi, Fabio Coppedè, Jessica Ponti, Claudia Placidi, Magda Blosi, Simona Ortelli, Anna Luisa Costa, and Lucia Migliore
Mutagenesis, Apr 2016; 10.1093/mutage/gew015
http://mutage.oxfordjournals.org/cgi/content/abstract/gew015v1

“Unique epigenetic influence of H2AX phosphorylation and H3K56
acetylation on normal stem cell radioresponses”
“Keith M. Jacobs, Sandeep Misri, Barbara Meyer, Suyash Raj, Cheri L. Zobel, Barry P. Sleckman, Dennis E. Hallahan, and Girdhar G. Sharma”
Mol. Biol. Cell, Apr 2016; 27: 1332 – 1345
http://www.molbiolcell.org/cgi/content/abstract/27/8/1332

“A ubiquitylation site in Cockayne syndrome B required for repair of
oxidative DNA damage, but not for transcription-coupled nucleotide excision repair”
Michael Ranes, Stefan Boeing, Yuming Wang, Franziska Wienholz, Hervé Menoni, Jane Walker, Vesela Encheva, Probir Chakravarty, Pierre-Olivier Mari, Aengus Stewart, Giuseppina Giglia-Mari, Ambrosius P. Snijders, Wim Vermeulen, and Jesper Q. Svejstrup
Nucleic Acids Res., Apr 2016; 10.1093/nar/gkw216.
http://nar.oxfordjournals.org/cgi/content/abstract/gkw216v2

Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo

Dylan McLaughlin, Christopher T. Coey, Wei-Chih Yang, Alexander C. Drohat, and Michael J. Matunis
J. Biol. Chem., Apr 2016; 291: 9014 – 9024
http://www.jbc.org/cgi/content/abstract/291/17/9014

“Long noncoding RNA POU6F2-AS2 is associated with oesophageal
squamous cell carcinoma”

Jing Liu, Xiangdong Sun, Hongcheng Zhu, Qin Qin, Xi Yang, and Xinchen Sun
J. Biochem., Apr 2016; 10.1093/jb/mvw025.
http://jb.oxfordjournals.org/cgi/content/abstract/mvw025v2

“Methylation of histone H4 lysine 20 by PR-Set7 ensures the integrity of late replicating sequence domains in Drosophila”
Yulong Li, Robin L. Armstrong, Robert J. Duronio, and David M. MacAlpine
Nucleic Acids Res., Apr 2016; 10.1093/nar/gkw333
http://nar.oxfordjournals.org/cgi/content/abstract/gkw333v1

Catalog # 4250-050-K includes :
Catalog Number Description Qty
4250-050-01 CometAssay® Lysis Solution 1
4250-050-02 CometAssay® LMAgarose 1
4250-050-03 CometSlides™ (2 Well, 25 Slides) 1
4250-050-04 200 mM EDTA, pH 10 1