CometAssay® Kit (2 x 20 well slides)

CometAssay® HT Kit includes two specially treated CometSlides™ (20 Well) and sufficient reagents (Lysis Solution, LMAgarose, and 200 mM EDTA pH 8) for 40 test samples.

Available Size(s): 40 Samples Catalog Number: 4252-040-K Category:

Description

FEATURES:

  • Specially treated slides to promote agarose adherence
  • Ready to use low melt agarose (LMAgarose) in convenient size
  • Lysis Solution suitable for either alkaline or neutral comet assay
  • Optimized electrophoresis conditions

APPLICATIONS:

  • Applicable for the analysis of either single- or double-strand DNA breaks
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens

Protocol(s)

protocol_4252-040-K

Material Safety Data Sheet(s)

msds_4250-050-02 LMAgarose

msds_4250-050-04 200mM EDTA

msds_4250-010-01 Lysis Solution

4252-040-01 Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 4252-040-K includes :
Catalog Number Description Qty
4250-010-01 CometAssay® Lysis Solution 1
4250-050-02 CometAssay® LMAgarose 1
4250-050-04 200 mM EDTA, pH 10 1
4252-040-01 CometSlides™  (20 Well, 2 Slides) 1

 

A role for the p53 tumour suppressor in regulating the balance between homologous recombination and non-homologous end joining
Sylvie Moureau, Janna Luessing, Emma Christina Harte, Muriel Voisin, and Noel Francis Lowndes
Open Bio, Sep 2016; 6: 160225.
http://rsob.royalsocietypublishing.org/cgi/content/abstract/6/9/160225

Special AT-rich Sequence-binding Protein 1 (SATB1) Functions as an Accessory Factor in Base Excision Repair

Simran Kaur, Yan Coulombe, Zubaidah M. Ramdzan, Lam Leduy, Jean-Yves Masson, and Alain Nepveu
J. Biol. Chem., Oct 2016; 291: 22769 – 22780
http://www.jbc.org/cgi/content/abstract/291/43/22769

Human CDK18 promotes replication stress signaling and genome stability
Giancarlo Barone, Christopher J. Staples, Anil Ganesh, Karl W. Patterson, Dominic P. Bryne, Katie N. Myers, Abhijit A. Patil, Claire E. Eyers, Sarah Maslen, J. Mark Skehel, Patrick A. Eyers, and Spencer J. Collis
Nucleic Acids Res., Oct 2016; 44: 8772 – 8785
http://nar.oxfordjournals.org/cgi/content/abstract/44/18/8772

A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1
“Yingfei Wang, Ran An, George K. Umanah, Hyejin Park, Kalyani Nambiar, Stephen M. Eacker, BongWoo
Kim, Lei Bao, Maged M. Harraz, Calvin Chang, Rong Chen, Jennifer E. Wang, Tae-In Kam, Jun Seop
Jeong, Zhi Xie, Stewart Neifert, Jiang Qian, Shaida A. Andrabi, Seth Blackshaw, Heng Zhu, Hongjun Song,
Guo-li Ming, Valina L. Dawson, and Ted M. Dawson”
Science, Oct 2016; 354: aad6872.
http://science.sciencemag.org/cgi/content/abstract/354/6308/aad6872

Amplification of TLK2 Induces Genomic Instability via Impairing the G2–M Checkpoint
Jin-Ah Kim, Meenakshi Anurag, Jamunarani Veeraraghavan, Rachel Schiff, Kaiyi Li, and Xiao-Song Wang
Mol. Cancer Res., Oct 2016; 14: 920 – 927
http://mcr.aacrjournals.org/cgi/content/abstract/14/10/920

Long noncoding RNA POU6F2-AS2 is associated with oesophageal squamous cell carcinoma
“Jing Liu, Xiangdong Sun, Hongcheng Zhu, Qin Qin, Xi Yang, and Xinchen Sun”
J. Biochem., Oct 2016; 160: 195 – 204.
http://jb.oxfordjournals.org/cgi/content/abstract/160/4/195

Lurbinectedin Specifically Triggers the Degradation of Phosphorylated RNA Polymerase II and the Formation of DNA Breaks in Cancer Cells
Gema Santamaría Nuñez, Carlos Mario Genes Robles, Christophe Giraudon, Juan Fernando Martínez-Leal, Emmanuel Compe, Frédéric Coin, Pablo Aviles, Carlos María Galmarini, and Jean-Marc Egly
Mol. Cancer Ther., Oct 2016; 15: 2399 – 2412
http://mct.aacrjournals.org/cgi/content/abstract/15/10/2399

Two familial ALS proteins function in prevention/repair of transcription-associated DNA damage
Sarah J. Hill, Daniel A. Mordes, Lisa A. Cameron, Donna S. Neuberg, Serena Landini, Kevin Eggan, and David M. Livingston
PNAS, Nov 2016; 113: E7701 – E7709
http://www.pnas.org/cgi/content/abstract/113/48/E7701

Epigenetic effects of carbon nanotubes in human monocytic cells “Deniz Öner, Matthieu Moisse, Manosij Ghosh, Radu C. Duca, Katrien Poels, Katrien Luyts, Eveline
Putzeys, Stevan M. Cokic, Kirsten Van Landuyt, Jeroen Vanoirbeek, Diether Lambrechts, Lode Godderis,
and Peter H.M. Hoet”
Mutagenesis, Nov 2016; 10.1093/mutage/gew053
http://mutage.oxfordjournals.org/cgi/content/abstract/gew053v1

“Distribution, quantification and toxicity of cinnamaldehyde in
electronic cigarette refill fluids and aerosols”

“Rachel Z Behar, Wentai Luo, Sabrina C Lin, Yuhuan Wang, Jackelyn Valle, James F Pankow, and Prue
Talbot”
Tob. Control, Nov 2016; 25: ii94 – ii102
http://tobaccocontrol.bmj.com/cgi/content/abstract/25/Suppl_2/ii94