CometChip®, 30 micron

$225.00

The CometChip® is an array of spatially encoded micropores (30 micron width), patterned on agarose. 96 separate macrowells, each containing ~400 micropores, are created by inserting the CometChip® into the a magnetically sealable cassette (96-Well CometChip® System).

Available Size(s): 1 CometChip Catalog Number: 4260-096-01 Category:

Description

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Single-cell capture

  • Facilitates automated imaging
  • No overlapping comets
  • Easy definition between head and tail
  • Minimize Cell Stress – Gravity capture of single-cells
  • Reduces cell shearing with wash and treatment steps
  • Avoids time-consuming trypsin treatment

Protocol(s)

Part of below Kit(s)

protocol_4260-096-ESK

Material Safety Data Sheet(s)

msds_4260-096-01 CometChip 30 micron

Presentation(s)

SOT 2017 Trevigen’s CometChip® Platform for High-Through-Put DNA Damage Analysis

CometChip® (96 Well)

Sykora, Peter et al. “Next Generation High Throughput DNA Damage Detection Platform for Genotoxic Compound Screening.” Scientific Reports 8:2771 (2018): 1-20.

Ge, Jing et al. “Micropatterned Comet Assay Enables High Throughput and Sensitive DNA Damage Quantification.” Mutagenesis 30.1 (2015): 11–19.

Li, Na et al. “Influenza Infection Induces Host DNA Damage and Dynamic DNA Damage Responses during Tissue Regeneration.” Cellular and molecular life sciences : CMLS 72.15 (2015): 2973–2988.

Ge, Jing et al. “CometChip: A High-Throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells.” Journal of Visualized Experiments : JoVE 92 (2014): 50607.

Sotiriou, Georgios A. et al. “Engineering Safer-by-Design, Transparent, Silica-Coated ZnO Nanorods with Reduced DNA Damage Potential.” Environmental science. Nano 1.2 (2014): 144–153.

Watson, Christa et al. “High-Throughput Screening Platform for Engineered Nanoparticle-Mediated Genotoxicity Using CometChip Technology.” ACS Nano 8.3 (2014): 2118–2133.

Ge, Jing et al. “Standard Fluorescent Imaging of Live Cells Is Highly Genotoxic.” Cytometry. Part A : the journal of the International Society for Analytical Cytology 83.6 (2013): 552–560.

Weingeist, David M. et al. “Single-Cell Microarray Enables High-Throughput Evaluation of DNA Double-Strand Breaks and DNA Repair Inhibitors.” Cell Cycle 12.6 (2013): 907–915.

Chao, Ming-Wei et al. “Genotoxicity of 2,6- and 3,5-Dimethylaniline in Cultured Mammalian Cells: The Role of Reactive Oxygen Species.” Toxicological Sciences 130.1 (2012): 48–59.

Mutamba, James T. et al. “XRCC1 and Base Excision Repair Balance in Response to Nitric Oxide.” DNA repair 10.12 (2011): 1282–1293.

Wood, David K. et al. “Single Cell Trapping and DNA Damage Analysis Using Microwell Arrays.” Proceedings of the National Academy of Sciences of the United States of America 107.22 (2010): 10008–10013.