This ELISA based assay detects poly (ADP-ribose) deposited by Tankyrase 1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with Tankyrase 1 activity.
- Chemiluminescent, non-radioactive format
- Higher throughput
- 96 test size
- Sensitivity down to 0.1 mUnits of Tankyrase 1.
For the screening of candidate Tankyrase 1 inhibitors and determination of IC50 values.