Tankyrase 1 (PARP5A) Activity Chemiluminescent Assay Kit

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The Tankyrase 1 Activity Chemiluminescent Assay Kit is for the in vitro screening of candidate Tankyrase 1 inhibitors and determination of IC50 values.

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Available Size(s): 192 Tests, 96 tests Catalog Number: 4701-096-K Category:

Description

This ELISA based assay detects poly (ADP-ribose) deposited by Tankyrase 1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with Tankyrase 1 activity.

FEATURES:

  • Chemiluminescent, non-radioactive format
  • Higher throughput
  • 96 test size
  • Sensitivity down to 0.1 mUnits of Tankyrase 1.

APPLICATIONS:
For the screening of candidate Tankyrase 1 inhibitors and determination of IC50 values.

Protocol(s)

protocol_4701-096-K

Material Safety Data Sheet(s)

msds_4675-096-01 PARP PeroxyGlowA

msds_4675-096-02 PARP PeroxyGlowB

msds_4700-096-01 Tankyrase 1 Enzyme 10 mUnits per ul

msds_4700-096-02 Assay Substrate

msds_4700-096-03 XAV939 1 mM

msds_4684-096-04 Anti-PAR monoclonal Antibody

msds_4684-096-05 Goat anti-mouse IgG-HRP

msds_4684-096-07 20X I-PAR Assay Buffer

msds_4684-096-03 5X Antibody Diluent

4685-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 4701-192-K includes :
Catalog Number Description Qty
4684-096-04 Anti-PAR monoclonal Antibody 2
4684-096-05 Goat anti-mouse IgG-HRP 2
4684-096-07 20X I-PAR Assay Buffer 2
4700-096-01 Tankyrase 1 Enzyme, 10 mUnits/µl 2
4700-096-02 Assay Substrate 2
4700-096-03 XAV939, 1 mM 2
4675-096-01 PARP PeroxyGlow™ A 2
4675-096-02 PARP PeroxyGlow™ B 2
4684-096-03 5X Antibody Diluent 2
4685-096-P Histone-Coated White Strip Well Plate, I-PAR 2
Catalog # 4701-096-K includes :
Catalog Number Description Qty
4684-096-04 Anti-PAR monoclonal Antibody 1
4684-096-05 Goat anti-mouse IgG-HRP 1
4684-096-07 20X I-PAR Assay Buffer 1
4700-096-01 Tankyrase 1 Enzyme, 10 mUnits/µl 1
4700-096-02 Assay Substrate 1
4700-096-03 XAV939, 1 mM 1
4675-096-01 PARP PeroxyGlow™ A 1
4675-096-02 PARP PeroxyGlow™ B 1
4684-096-03 5X Antibody Diluent 1
4685-096-P Histone-Coated White Strip Well Plate, I-PAR 1