PARP Universal Colorimetric Assay Kit

$489.00

The PARP Universal Colorimetric Assay Kit is ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Available Size(s): 96 tests Catalog Number: 4677-096-K Category:

Description

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

FEATURES:

  • Colorimetric readout
  • 96 strip-well format
  • Sensitive – detects 10 mU PARP /well
  • Assay Time ~3 hrs

APPLICATIONS:
For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Protocol(s)

protocol_4677-096-K

Material Safety Data Sheet(s)

msds_4671-096-02 20X PARP Buffer

msds_4671-096-03 10X PARP Cocktail

msds_4671-096-06 10X Activated DNA

msds_4668-050-01 PARP HSA Enzyme

msds_4667-50-03 3-Aminobenzamide

msds_4671-096-04 10X Strep-Diluent

4677-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4822-96-08 TACS-Sapphire

msds_4800-30-06 Strep-HRP

Catalog # 4677-096-K includes :
Catalog Number Description Qty
4667-50-03 3-Aminobenzamide 1
4668-050-01 PARP-HSA, 10 Units/µl 1
4671-096-02 20X PARP Buffer 2
4671-096-03 10X PARP Cocktail 1
4671-096-06 10X Activated DNA 1
4671-096-04 10X Strep-Diluent 1
4677-096-P Histone-Coated Plate Natural Strip Well Plate 1
4800-30-06 Strep-HRP 1
4822-96-08 TACS-Sapphire™ 1

Effect of Small-Molecule Modification on Single-Cell Pharmacokinetics of PARP Inhibitors
Greg M. Thurber, Thomas Reiner, Katherine S. Yang, Rainer H. Kohler, and Ralph Weissleder
Mol. Cancer Ther., Apr 2014; 13: 986 – 995
http://mct.aacrjournals.org/cgi/content/abstract/13/4/986

Combinatorial Effects of PARP Inhibitor PJ34 and Histone Deacetylase Inhibitor Vorinostat on Leukemia Cell Lines
EWA JASEK, MARIUSZ GAJDA, GRZEGORZ J. LIS, MAŁGORZATA JASIŃSKA, and JAN A. LITWIN
Anticancer Res, Apr 2014; 34: 1849 – 1856.
http://ar.iiarjournals.org/cgi/content/abstract/34/4/1849

Silencing of poly(ADP-ribose) polymerase-1 suppresses hyperstretch-induced expression of inflammatory cytokines in vitro
Wang, J., Liu, L., Xia, Y. and Wu, D.
Acta Biochim Biophys Sin, May 2014; 10.1093/abbs/gmu035.
http://abbs.oxfordjournals.org/cgi/content/abstract/gmu035v1

New Insights into PARP Inhibitors’ Effect on Cell Cycle and Homology- Directed DNA Damage Repair
Petar Jelinic and Douglas A. Levine
Mol. Cancer Ther., Jun 2014; 13: 1645 – 1654.
http://mct.aacrjournals.org/cgi/content/abstract/13/6/1645

Disruption of Wnt/β-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells

“Ozlem Kulak, Hua Chen, Brody Holohan, Xiaofeng Wu, Huawei He, Dominika Borek, Zbyszek
Otwinowski, Kiyoshi Yamaguchi, Lauren A. Garofalo, Zhiqiang Ma, Woodring Wright, Chuo Chen, Jerry W.Shay, Xuewu Zhang, and Lawrence Lum”
Mol. Cell. Biol., Jul 2015; 35: 2425 – 2435.
http://mcb.asm.org/cgi/content/abstract/35/14/2425

Acyl-CoA-binding domain containing 3 modulates NAD+ metabolism through activating poly(ADP-ribose) polymerase 1

Yong Chen, Sookhee Bang, Soohyun Park, Hanyuan Shi, and Sangwon F. Kim
Biochem. J., Jul 2015; 469: 189 – 198.
http://www.biochemj.org/cgi/content/abstract/469/2/189