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PARP Homogeneous Inhibition Assay Kit

$495.00

The PARP Homogeneous Inhibition Assay Kit is for the high-throughput in vitro screening of candidate PARP-1 inhibitors and determination of relative IC50 values.

Contract Services are also available.

Available Size(s): 96 tests Catalog Number: 4690-096-K Category:

Product Description

4690-096-k_calculation_worksheetv2

This homogeneous assay couples PARP-1 autoribosylation to a fluorescent generating cycling assay. Inhibitors are identified by an increase in fluorescent signal when PARP-1 activity is inhibited.

FEATURES:

  • Highly sensitive fluorescent assay
  • Detect as little as 10% inhibition of PARP Activity
  • Inhibitors identified by increase in Fluorescent Signal
  • No wash steps
  • 96 test size
  • Rapid – Assay takes ~ 1.5 hr

APPLICATIONS:
For the screening of candidate PARP inhibitors and determination of relative IC50 values.

Protocol(s)

protocol_4690-096-K

Material Safety Data Sheet(s)

msds_4690-096-01 10X Cycling Enzymes

msds_4690-096-03 PARP-HSA Enzyme 200 ng per ul

msds_4690-096-04 Activated DNA 200 ng per ul

msds_4690-096-05 10X Resazurin

msds_4690-096-06 10X Buffer

msds_4690-096-07 Stop Solution

msds_4690-096-08 2 NAD

4690-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

Catalog # 4690-096-K includes :
Catalog Number Description Qty
4690-096-01 10X Cycling Enzymes 1
4690-096-08 2 µM NAD 1
4690-096-03 PARP 1 Enzyme 1
4690-096-04 Activated DNA 1
4690-096-05 10X Resazurin 1
4690-096-06 10X Buffer 1
4690-096-07 Stop Solution 1
4690-096-P 96-well Black Microplate 1

Insights into PARP Inhibitors’ Selectivity Using Fluorescence Polarization and Surface Plasmon Resonance Binding Assays
Gianluca Papeo, Nilla Avanzi, Serena Bettoni, Antonella Leone, Mauro Paolucci, Rita Perego, Francesca Quartieri, Federico Riccardi-Sirtori, Sandrine Thieffine, Alessia Montagnoli, and Rosita Lupi
J Biomol Screen, Jun 2014; 10.1177/1087057114538319.
http://jbx.sagepub.com/cgi/content/abstract/1087057114538319v1