This homogeneous assay couples PARP-1 autoribosylation to a fluorescent generating cycling assay. Inhibitors are identified by an increase in fluorescent signal when PARP-1 activity is inhibited.
- Highly sensitive fluorescent assay
- Detect as little as 10% inhibition of PARP Activity
- Inhibitors identified by increase in Fluorescent Signal
- No wash steps
- 96 test size
- Rapid – Assay takes ~ 1.5 hr
For the screening of candidate PARP inhibitors and determination of relative IC50 values.