PARP/Apoptosis Colorimetric Assay Kit

$544.00

The PARP/Apoptosis Colorimetric Assay Kit is ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Available Size(s): 96 tests Catalog Number: 4684-096-K Category:

Description

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.  During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

FEATURES:

  • Colorimetric readout
  • 96 well format
  • Highly sensitive – detects 0.1 mU PARP ~500 cells
  • Dynamic range between 0.1 to 10 mU PARP
  • Requires 10-100 ng extract for detection
  • Assay Time ~3 hrs

APPLICATIONS:

  • Measure PARP-1 activity in cell extracts before and after apoptosis.
  • For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Protocol(s)

protocol_4684-096-K

Material Safety Data Sheet(s)

msds_4684-096-01 PARP-HSA 10 mUnits per

msds_4684-096-02 20 mM NAD

msds_4684-096-03 5X Antibody Diluent

msds_4684-096-04 Anti-PAR monoclonal Antibody

msds_4684-096-05 Goat anti-mouse IgG-HRP

msds_4684-096-06 10 mM Etoposide

msds_4684-096-07 20X I-PAR Assay Buffer

4684-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4671-096-06 10X Activated DNA

msds_4822-96-08 TACS-Sapphire

Catalog # 4684-096-K includes :
Catalog Number Description Qty
4671-096-06 10X Activated DNA 1
4684-096-01 PARP-HSA, 10 mUnits/µl 1
4684-096-02 20 mM NAD 1
4684-096-04 Anti-PAR monoclonal Antibody 1
4684-096-05 Goat anti-mouse IgG-HRP 1
4684-096-06 10 mM Etoposide 1
4684-096-07 20X I-PAR Assay Buffer 1
4684-096-03 5X Antibody Diluent 1
4684-096-P Histone-Coated Natural Strip Well Plate, I-PAR 1
4822-96-08 TACS-Sapphire 1

“Ultraviolet Radiation Signaling through TLR4/MyD88
Constrains DNA Repair and Plays a Role in Cutaneous
Immunosuppression”
Erin Harberts, Hua Zhou, Rita Fishelevich, Juan Liu, and Anthony A. Gaspari
J. Immunol., Apr 2015; 194: 3127 – 3135.
http://www.jimmunol.org/cgi/content/abstract/194/7/3127

“Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose
Polymerase-Dependent Cell Death Pathways and Protects against
Excitotoxic and Seizure-Induced Neuronal Injury”

“Beatrice D’Orsi, Tobias Engel, Shona Pfeiffer, Saheli Nandi, Thomas Kaufmann, David C. Henshall, and
Jochen H. M. Prehn”
J. Neurosci., Apr 2016; 36: 4564 – 4578
http://www.jneurosci.org/cgi/content/abstract/36/16/4564

NAD+ repletion improves muscle function in muscular dystrophy and counters global PARylation

“Dongryeol Ryu, Hongbo Zhang, Eduardo R. Ropelle, Vincenzo Sorrentino, Davi A. G. Mázala, Laurent Mouchiroud, Philip L. Marshall, Matthew D. Campbell, Amir Safi Ali, Gary M. Knowels, Stéphanie Bellemin, Shama R. Iyer, Xu Wang, Karim Gariani, Anthony A. Sauve, Carles Cantó, Kevin E. Conley, Ludivine Walter, Richard M. Lovering, Eva R. Chin, Bernard J. Jasmin, David J. Marcinek, Keir J. Menzies, and Johan Auwerx”
Science Translational Medicine, Oct 2016; 8: 361ra139
http://stm.sciencemag.org/cgi/content/abstract/8/361/361ra139