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PARP/Apoptosis Chemiluminescent Assay Kit

$544.00

The PARP/Apoptosis Chemiluminescent Assay Kit is ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Contract Services are also available.

Available Size(s): 96 tests Catalog Number: 4685-096-K Category:

Product Description

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity.  During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

FEATURES:

  • Chemiluminescent readout
  • 96 well format
  • Highly sensitive – detects 0.1 mU PARP ~500 cells
  • Dynamic range between 0.1 to 10 mU PARP
  • Requires 10-100 ng extract for detection
  • Assay Time ~3 hrs

APPLICATIONS:

  • Measure PARP-1 activity in cell extracts before and after apoptosis.
  • For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Protocol(s)

protocol_4685-096-K

Material Safety Data Sheet(s)

msds_4684-096-01 PARP-HSA 10 mUnits per

msds_4684-096-02 20 mM NAD

msds_4684-096-03 5X Antibody Diluent

msds_4684-096-04 Anti-PAR monoclonal Antibody

msds_4684-096-05 Goat anti-mouse IgG-HRP

msds_4684-096-06 10 mM Etoposide

msds_4684-096-07 20X I-PAR Assay Buffer

4685-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4675-096-01 PARP PeroxyGlowA

msds_4675-096-02 PARP PeroxyGlowB

msds_4671-096-06 10X Activated DNA

Catalog # 4685-096-K includes :
Catalog Number Description Qty
4671-096-06 10X Activated DNA 1
4684-096-01 PARP-HSA, 10 mUnits/µl 1
4684-096-02 20 mM NAD 1
4684-096-04 Anti-PAR monoclonal Antibody 1
4684-096-05 Goat anti-mouse IgG-HRP 1
4684-096-06 10 mM Etoposide 1
4684-096-07 20X I-PAR Assay Buffer 1
4675-096-01 PARP PeroxyGlow™ A 1
4675-096-02 PARP PeroxyGlow™ B 1
4684-096-03 5X Antibody Diluent 1
4685-096-P Histone-Coated White Strip Well Plate, I-PAR 1