This homogeneous assay couples PARG hydrolysis of PAR polymer to a light generating luciferase assay. Inhibitors are identified by a decrease in luminescent signal when PARG activity is inhibited.
- Rapid 30-minute assay
- Homogeneous assay – No wash steps
- Highly sensitive luminescent assay, detects as little as 10% PARG inhibition
For the screening of candidate PARG inhibitors and determination of IC50 values.