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PARG Universal Chemiluminescent Assay Kit

$533.00

The PARG Universal Chemiluminescent Assay Kit is ideal for the in vitro screening of candidate PARG inhibitors and determination of IC50 values.

Contract Services are also available.

Available Size(s): 96 tests Catalog Number: 4682-096-K Category:

Product Description

This ELISA based assay measures the loss of biotinylated poly (ADP-ribose) from immobilized histones in a 96-well format due to PARG activity. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARG activity.

FEATURES:

  • Chemiluminescent readout
  • 96 well format
  • Highly sensitive – detects 50 pg PARG per well
  • Assay Time ~2 hrs

APPLICATIONS:
For the in vitro screening of candidate PARG inhibitors and determination of IC50 values.

Protocol(s)

protocol_4682-096-K

Material Safety Data Sheet(s)

msds_4680-096-01 PARG Enzyme

msds_4680-096-02 10X PARG Buffer

msds_4680-096-03 100 mM DEA

msds_4671-096-02 20X PARP Buffer

msds_4671-096-03 10X PARP Cocktail

msds_4671-096-06 10X Activated DNA

msds_4668-050-01 PARP HSA Enzyme

4678-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4675-096-01 PARP PeroxyGlowA

msds_4675-096-02 PARP PeroxyGlowB

msds_4800-30-06 Strep-HRP

Catalog # 4682-096-K includes :
Catalog Number Description Qty
4668-050-01 PARP-HSA, 10 Units/µl 1
4671-096-02 20X PARP Buffer 1
4671-096-03 10X PARP Cocktail 1
4671-096-06 10X Activated DNA 1
4680-096-01 PARG Enzyme (1µg/ml) 1
4680-096-03 DEA 1
4675-096-01 PeroxyGlow A 1
4675-096-02 PeroxyGlow B 1
4678-096-P 96-Well Histone Coated Plate (stripwell) (white) 1
4680-096-02 10X PARG Buffer 1
4800-30-06 Streptavidin-HRP 1