This ELISA based assay measures the loss of biotinylated poly (ADP-ribose) from immobilized histones in a 96-well format due to PARG activity. The addition of Strep-HRP (biotin-binding protein) and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARG activity.
- Chemiluminescent readout
- 96 well format
- Highly sensitive – detects 50 pg PARG per well
- Assay Time ~2 hrs
For the in vitro screening of candidate PARG inhibitors and determination of IC50 values.