Anti-PAR Monoclonal Antibody


This monoclonal antibody is specific for poly(ADP-ribose) (PAR) polymer. Trevigen’s PAR-PARP Western Control Protein may be used as positive controls for ELISA and Western blot analysis.

Available Size(s): 100 μl Catalog Number: 4335-MC-100 Category:


1 mg/ml in 1X PBS containing 50% glycerol.
This monoclonal antibody is an IgG3 purified from mouse ascites.
Purified ADP-ribose polymers between 2 and 50 units long.
PAR polymers 2 to 50 units long
Store antibody at -20°C in a manual defrost freezer.
ELISA, Western Blot Analysis, immunoprecipitation, and immunopurification



Material Safety Data Sheet(s)

msds_4335-MC-100 Anti-PAR Monoclonal

PARP1-TDP1 coupling for the repair of topoisomerase I-induced DNA damage,
Benu Brata Das, Shar-yin N. Huang, Junko Murai, Ishita Rehman, Jean-Christophe Amé, Souvik Sengupta, Subhendu K. Das, Papiya Majumdar, Hongliang Zhang, Denis Biard, Hemanta K. Majumder, Valérie Schreiber, and Yves Pommier
Nucleic Acids Res., Apr 2014; 42: 4435 – 4449.

DNA double-strand breaks promote methylation of histone H3 on lysine 9 and transient formation of repressive chromatin

Marina K. Ayrapetov, Ozge Gursoy-Yuzugullu, Chang Xu, Ye Xu, and Brendan D. Price
PNAS, Jun 2014; 111: 9169 – 9174.

Poly(ADP-ribose) Polymerase 1 (PARP1) Associates with E3 Ubiquitin- Protein Ligase UHRF1 and Modulates UHRF1 Biological Functions

“Mike De Vos, Rosy El Ramy, Delphine Quénet, Patricia Wolf, Fabio Spada, Najat Magroun, Federica
Babbio, Valérie Schreiber, Heinrich Leonhardt, Ian Marc Bonapace, and Françoise Dantzer”
J. Biol. Chem., Jun 2014; 289: 16223 – 16238.

Poly(ADP-Ribose) Polymerase-1 Causes Mitochondrial Damage and Neuron Death Mediated by Bnip3
Ping Lu, Amit Kamboj, Spencer B. Gibson, and Christopher M. Anderson
J. Neurosci., Nov 2014; 34: 15975 – 15987.

The 3′-5′ DNA Exonuclease TREX1 Directly Interacts with Poly(ADP-ribose) Polymerase-1 (PARP1) during the DNA Damage Response
Takuya Miyazaki, Yong-Soo Kim, Jeongheon Yoon, Hongsheng Wang, Teruhiko Suzuki, and Herbert C. Morse, III
J. Biol. Chem., Nov 2014; 289: 32548 – 32558.

Aag-initiated base excision repair promotes ischemia reperfusion injury in liver, brain, and kidney

Mohammad R. Ebrahimkhani, Ali Daneshmand, Aprotim Mazumder, Mariacarmela Allocca, Jennifer A. Calvo, Nona Abolhassani, Iny Jhun, Sureshkumar Muthupalani, Cenk
Ayata, and Leona D. Samson PNAS, Nov 2014; 111: E4878 – E4886.

Poly-ADP ribosylation of PTEN by tankyrases promotes PTEN degradation and tumor growth

Nan Li, Yajie Zhang, Xin Han, Ke Liang, Jiadong Wang, Lin Feng, Wenqi Wang, Zhou Songyang, Chunru Lin, Liuqing Yang, Yonghao Yu, and Junjie Chen
Genes & Dev., Jan 2015; 29: 157 – 170

SUMOylation Regulates Polo-like Kinase 1-interacting Checkpoint Helicase (PICH) during Mitosis
Vinidhra Sridharan, Hyewon Park, Hyunju Ryu, and Yoshiaki Azuma
J. Biol. Chem., Feb 2015; 290: 3269 – 3276.

The XRCC1 phosphate-binding pocket binds poly (ADP-ribose) and is
required for XRCC1 function”
“Claire Breslin, Peter Hornyak, Andrew Ridley, Stuart L. Rulten, Hana Hanzlikova, Antony W. Oliver, and
Keith W. Caldecott”
Nucleic Acids Res., Aug 2015; 43: 6934 – 6944.

“The PIN domain of EXO1 recognizes poly(ADP-ribose) in DNA damage

Feng Zhang, Jiazhong Shi, Shih-Hsun Chen, Chunjing Bian, and Xiaochun Yu
Nucleic Acids Res., Sep 2015; 10.1093/nar/gkv939.

“Poly(ADP-ribosyl)ation of Methyl CpG Binding Domain Protein 2
Regulates Chromatin Structure”

Annette Becker, Peng Zhang, Lena Allmann, Daniela Meilinger, Bianca Bertulat, Daniel Eck, Maria Hofstaetter, Giody Bartolomei, Michael O. Hottiger, Valérie Schreiber, Heinrich Leonhardt, and M. Cristina Cardoso
J. Biol. Chem., Mar 2016; 291: 4873 – 4881

“Distinct genetic control of homologous recombination repair of
Cas9-induced double-strand breaks, nicks and paired nicks”

Lianne E.M. Vriend, Rohit Prakash, Chun-Chin Chen, Fabio Vanoli, Francesca Cavallo, Yu Zhang, Maria Jasin, and Przemek M. Krawczyk
Nucleic Acids Res., Mar 2016; 10.1093/nar/gkw179.

p53 coordinates base excision repair to prevent genomic instability
Mattia Poletto, Arnaud J. Legrand, Sally C. Fletcher, and Grigory L. Dianov
Nucleic Acids Res., Apr 2016; 44: 3165 – 3175.

“Hyperthermia Synergizes with Chemotherapy by Inhibiting PARP1-Dependent DNA Replication Arrest”
“Lea Schaaf, Matthias Schwab, Christoph Ulmer, Simon Heine, Thomas E. Mürdter, Jens O. Schmid, Georg Sauer, Walter E. Aulitzky, and Heiko van der Kuip”
Cancer Res., May 2016; 76: 2868 – 2875.

“APE1/Ref-1 facilitates recovery of gray and white matter and
neurological function after mild stroke injury”

“R. Anne Stetler, Yanqin Gao, Rehana K. Leak, Zhongfang Weng, Yejie Shi, Lili Zhang, Hongjian Pu, Feng
Zhang, Xiaoming Hu, Sulaiman Hassan, Carolyn Ferguson, Gregg E. Homanics, Guodong Cao, Michael V.L. Bennett, and Jun Chen”
PNAS, Jun 2016; 113: E3558 – E3567.

“Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks”
“Lianne E.M. Vriend, Rohit Prakash, Chun-Chin Chen, Fabio Vanoli, Francesca Cavallo, Yu Zhang, Maria
Jasin, and Przemek M. Krawczyk”

“A Radiotracer Strategy to Quantify PARP-1 Expression In Vivo Provides a Biomarker That Can Enable Patient Selection for PARP
Inhibitor Therapy”

“Mehran Makvandi, Kuiying Xu, Brian P. Lieberman, Redmond-Craig Anderson, Samuel Sander Effron,
Harrison D. Winters, Chenbo Zeng, Elizabeth S. McDonald, Daniel A. Pryma, Roger A. Greenberg, and Robert H. Mach”
Cancer Res., Aug 2016; 76: 4516 – 4524.

“Nitric Oxide Suppresses β-Cell Apoptosis by Inhibiting the DNA
Damage Response”

“Bryndon J. Oleson, Katarzyna A. Broniowska, Aaron Naatz, Neil Hogg, Vera L. Tarakanova, and John A.Corbett”
Mol. Cell. Biol., Aug 2016; 36: 2067 – 2077.

A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response
Jay Oza, Bratati Ganguly, Atul Kulkarni, Vasudeva Ginjala, Ming Yao, and Shridar Ganesan
J. Biol. Chem., Oct 2016; 291: 22881 – 22893

“PARylation of the forkhead-associated domain protein DAWDLE regulates plant immunity”
Baomin Feng, Shisong Ma, Sixue Chen, Ning Zhu, Shuxin Zhang, Bin Yu, Yu Yu, Brandon Le, Xuemei Chen, Savithramma P Dinesh-Kumar, Libo Shan, and Ping He
EMBO Rep., Oct 2016; 10.15252/embr.201642486

“FASN regulates cellular response to genotoxic treatments by
increasing PARP-1 expression and DNA repair activity via NF- B and

“Xi Wu, Zizheng Dong, Chao J. Wang, Lincoln James Barlow, Valerie Fako, Moises A. Serrano, Yue Zou,
Jing-Yuan Liu, and Jian-Ting Zhang”
PNAS, Nov 2016; 113: E6965 – E6973