In an ELISA assay, immobilized gamma-H2AX antibody in the wells of a 96-well plate captures gamma-H2AX from sample lysate. Incubation with a H2AX detecting antibody, followed by addition of a goat anti-mouse HRP conjugate and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of gamma-H2AX in the sample. The gamma-H2AX polyclonal antibody may be purchased separately.
- Chemiluminescent, non-radioactive ELISA
- Pre-coated 96 well capture antibody plate
- Dynamic range from 10 pM to 800pM
- Sensitivity with 5 pM of gamma-H2AX
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.