gamma-H2AX Pharmacodynamic Assay Kit

$1,250.00

The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies.

Contract Services are also available.

Available Size(s): 96 tests Catalog Number: 4418-096-K Category:

Description

4418-096-k_calculation_worksheet

In an ELISA assay, immobilized gamma-H2AX antibody in the wells of a 96-well plate captures gamma-H2AX from sample lysate.  Incubation with a H2AX detecting antibody, followed by addition of a goat anti-mouse HRP conjugate and a chemiluminescent HRP substrate yields relative light units (RLU) that directly correlates with the amount of gamma-H2AX in the sample. The gamma-H2AX polyclonal antibody may be purchased separately.

FEATURES:

  • Chemiluminescent, non-radioactive ELISA
  • Pre-coated 96 well capture antibody plate
  • Dynamic range from 10 pM to 800pM
  • Sensitivity with 5 pM of gamma-H2AX

APPLICATIONS:
Quantitation of gamma-H2AX levels in peripheral blood mononuclear cells, tissue culture cells, and tissue biopsies.

Protocol(s)

protocol_4418-096-K

Material Safety Data Sheet(s)

msds_4418-096-01 Gamma-H2AX Standard

msds_4418-096-02 Assay Buffer

msds_4418-096-03 H2AX IgM Detecting Antibody

msds_4418-096-04 Goat anti-Mouse IgM HRP Conjugate

msds_4418-096-05 Cell Lysis Reagent

msds_4418-096-06 Jurkat Cell Lysate Control

msds_4418-096-07 25X Wash Buffer

4418-096-P Safety Data Sheets are not provided for “articles” defined as a manufactured item other than a fluid or particle.

msds_4675-096-01 PARP PeroxyGlowA

msds_4675-096-02 PARP PeroxyGlowB

Catalog # 4418-096-K includes :
Catalog Number Description Qty
4418-096-01 Gamma-H2AX Standard, 1 µM 1
4418-096-06 Jurkat Cell Lysate Control 1
4418-096-02 Assay Buffer 1
4418-096-05 Cell Lysis Reagent 1
4418-096-07 25X Wash Buffer 1
4418-096-P 96-Stripwell pre-coated plate with 3 film sealers 1
4675-096-01 PARP PeroxyGlow™ A 1
4675-096-02 PARP PeroxyGlow™ B 1
4418-096-03 H2AX IgM Detecting Antibody 1
4418-096-04 Goat anti-Mouse IgM HRP Conjugate 1

Abstract 2423: Development of a validated high throughput ELISA assay for gamma H2AX as a pharmacodynamic marker
Jay George and Hai Xu
Cancer Res., Oct 2014; 74: 2423.
http://cancerres.aacrjournals.org/cgi/content/abstract/74/19_Supplement/2423}

Engineered cellular gene-replacement platform for selective and inducible proteolytic profiling
Charles W. Morgan, Juan E. Diaz, Samantha G. Zeitlin, Daniel C. Gray, and James A. Wells
PNAS, Jul 2015; 112: 8344 – 8349.
http://www.pnas.org/cgi/content/abstract/112/27/8344