Anti-Phosphorylated Histone H2AX Affinity Purified Polyclonal Antibody

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The rabbit polyclonal antibody raised against a synthetic peptide may be used to detect phosphorylated histone H2AX by immunodetection which correlates with the presence of double-strand DNA breaks.

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Available Size(s): 100 ul, 20 ul Catalog Number: 4418-APC-020 Category:

Description

ANTIBODY CONCENTRATION
Provided at 0.5 mg/ml in 1X PBS containing 50% glycerol.
This rabbit polyclonal antibody is peptide affinity purified.
IMMUNOGEN
Synthetic phosphorylated peptide (CKATQA[pS]QEY)
SPECIFICITY
This antibody detects human and mouse gamma-H2AX.
STORAGE
Store antibody at -20°C in a manual defrost freezer.
APPLICATIONS
Western Blot Analysis and Immunocytochemistry.

Protocol(s)

protocol_4418-APC-020

protocol_4418-APC-100

Material Safety Data Sheet(s)

msds_4418-APC-020 anti-phos histone H2AX

msds_4418-APC-100 anti-phos histone H2AX

Failure of cell cleavage induces senescence in tetraploid primary cells
Andreas Panopoulos, Cristina Pacios-Bras, Justin Choi, Mythili Yenjerla, Mark A. Sussman, Rati Fotedar, and Robert L. Margolis
Mol. Biol. Cell, Oct 2014; 25: 3105 – 3118.
http://www.molbiolcell.org/cgi/content/abstract/25/20/3105

Micronucleus formation causes perpetual unilateral chromosome inheritance in mouse embryos
Cayetana Vázquez-Diez, Kazuo Yamagata, Shardul Trivedi, Jenna Haverfield, and Greg FitzHarris
PNAS, Jan 2016; 113: 626 – 631
http://www.pnas.org/cgi/content/abstract/113/3/626