TACS•XL® Basic Kit

The TACS•XL® Basic Kit provides the reagents necessary for routine labeling, including two permeabilization reagents, labeling buffers and reagents, Strep-HRP and TACS-Nuclease™ reagents for generating positive controls with your own samples. It is ideal for researchers whose labs are equipped for standard immunohistochemical procedures involving horseradish-peroxidase or who want to adapt the assay for fluorescence detection. Add-on TACS•XL® Blue Label Module and TACS•XL® DAB Module detection modules are available separately.

Available Size(s): 30 Samples Catalog Number: 4828-30-K Categories: ,

Description

TACS•XL® embodies a more robust approach for the in situ detection of apoptosis than other methods. The TACS•XL® Kits are based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis. The incorporation of BrdU by TdT is more efficient than either biotinylated or digoxigenin labeled nucleotides used in other terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based assays. The detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. The combination of antibody specificity with the signal enhancing properties of biotin streptavidin results in precise cellular labeling and the highest signal-to-noise ratio observed in competitive testing.

The TACS•XL® Basic Kit provides the reagents necessary for routine labeling, including two permeabilization reagents, labeling buffers and reagents, Strep-HRP and TACS-Nuclease™ reagents for generating positive controls with your own samples. It is ideal for researchers whose labs are equipped for standard immunohistochemical procedures involving horseradish-peroxidase want to adapt the assay for fluorescence detection.  The kit doesn’t not contain any peroxydase-substrate or counterstain. Add-on TACS•XL® Blue Label Module and TACS•XL® DAB Module detection modules are available separately.

Please refer to our components listing for details.

 

Protocol(s)

protocol_4828-30-K

Material Safety Data Sheet(s)

msds_4800-30-01 Proteinase K

msds_4800-30-06 Strep-HRP

msds_4810-30-02 10X TdT Labeling Buffer

msds_4810-30-03 10X TdT Stop Buffer

msds_4810-30-05 TdT Enzyme

msds_4828-30-04 B dNTP Mix

msds_4828-30-06 Anti-BrdU Antibody

msds_4828-30-12 Strep-Diluent

msds_4876-05-01 Cytonin

Catalog # 4828-30-K includes :
Catalog Number Description Qty
4800-30-01 Proteinase K 1
4800-30-06 Streptavidin-HRP 1
4810-30-02 TACS® 2 TdT Labeling Buffer 1
4810-30-03 TACS® 2 TdT Stop Buffer 1
4810-30-05 TdT Enzyme 1
4828-30-04  B-dNTP Mix 1
4828-30-06 anti-BrdU antibody 1
4828-30-12 Strep-Diluent 1
4876-05-01 Cytonin™ 1