TACS•XL® embodies a more robust approach for the in situ detection of apoptosis than other methods. The TACS•XL® Kits are based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that are formed during apoptosis. The incorporation of BrdU by TdT is more efficient than either biotinylated or digoxigenin labeled nucleotides used in other terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-based assays. The detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. The combination of antibody specificity with the signal enhancing properties of biotin streptavidin results in precise cellular labeling and the highest signal-to-noise ratio observed in competitive testing.
Please refer to our components listing for details.