The extracellular scaffolding in skin tissue can make it problematic to permeabilize and high background is a common problem with skin samples. The DermaTACS™ Kit includes two permeabilization reagents as options for permeabilization along with a detailed protocol with hints and tips for optimal labeling of skin samples based on empirical testing. Low background is achieved using a combination of the high specificity of Trevigen’s unique cation-based optimized TdT labeling reaction using Apoptosis Grade™ TdT and TACS Blue Label™ as the visualization method. Also included is TACS-Nuclease™ to generate a positive control from your own sample. In conjunction with the excellent contrast between the TACS Blue Label™ and the Red Counterstain C, the positive control ensures easy data interpretation.
This complete kit provides all the reagents required for labeling including two permeabilization reagents, labeling and detection reagents, stop buffer, counterstain, and TACS-Nuclease™.